Product Name:
Myb-pS532
Product Number:
ab-pn627
Target Full Name: Transcriptional activator Myb
Target Alias: C-myb; v-myb myeloblastosis viral oncogene
Product Type Specific: Myb phosphosite-specific antibody
Antibody Code: PN627
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S532
Protein UniProt: P10242
Protein SigNET: Myb
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human Myb
Antibody Immunogen Sequence: QEVE(pS)PTD(βA)C
Antibody Immunogen Description: Corresponds to amino acid residues Q528 to D535. S532 phosphorylation inhibits transcriptional activity by promoting interaction with a putative inhibitory factor resulting in decreased Myb activity. This is one of the major in vivo phosphorylation sites in Myb (≥11 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus). Myb is known to be phosphorylated at this site in vitro by ERK1 (MAPK3).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus macaque | Dog | Rat | Mouse | Platypus | Chicken | Frog.
Scientific Background: Myb (Transcriptional activator Myb) is a master transcription factor critical for regulating hematopoietic cell proliferation, differentiation, and survival, functioning as a key regulator in stem and progenitor cells. In vertebrates, Myb is crucial for hematopoiesis, while in plants, it regulates metabolism, development, and stress responses. It features a conserved N-terminal DNA-binding domain (DBD) with tandem repeats, a central activation domain, and a C-terminal negative regulatory domain. The three tandem repeats (R1,R2,R3), each containing three tryptophan residues, allowing it to bind to specific DNA sequences ( 5'-YAAC[GT]G-3'), and it act as a transcription factor, often in conjunction with co-activators like p300/CBP. c-Myb is regulated through phosphorylation, ubiquitination, and proteasome degradation. c-Myb is crucial for normal hematopoiesis, as its deficiency causes severe, fatal anemia in mice due to lack of fetal hepatic erythropoiesis. It is negatively regulated by microRNA-155 (miR-155). It is expressed in bone marrow, breast, intestine, and lymphoid tissue. The c-Myb protein can be converted into an oncogenic (cancer-causing) v-Myb protein through specific mutations or deletions, which are often implicated in leukemia and solid cancers. This description may include information annotated by UniProt and/or Google AI.