Product Name:
MEF2D-pS180
Product Number:
ab-pn625
Target Full Name: Myocyte-specific enhancer factor 2D
Target Alias: MEFD; myocyte enhancer factor 2D
Product Type Specific: MEF2D phosphosite-specific antibody
Antibody Code: PN625
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S180
Protein UniProt: Q14814
Protein SigNET: MEF2D
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human MEF2D
Antibody Immunogen Sequence: PRLL(pS)PQQ(βA)C
Antibody Immunogen Description: Corresponds to amino acid residues P176 to Q183. S180 phosphorylation stimulates transcriptional activity. This is the major in vivo phosphorylation site in MEF2D (≥51 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus). MEF2D is known to be phosphorylated at this site in vitro by ERK5 (MAPK7).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rat | Mouse | Platypus | Frog | Zebra fish.
Related Product 1: MEF2A T108 phosphorylation antibody (Cat. No.: AB-PN622)
Related Product 2: MEF2C S396 phosphorylation antibody (Cat. No.: AB-PN623)
Related Product 3: MEF2D S121 phosphorylation antibody (Cat. No.: AB-PN624)
Scientific Background: MEF2D (Myocyte-specific enhancer factor 2D) is a transcriptional activator that belongs to the MADS-box family. It binds specifically to the MEF2 element, 5'-YTA[AT](4)TAR-3', found in numerous muscle-specific, growth factor- and stress-induced genes. It controls muscle and neuronal cell differentiation and acts as a survival factor, particularly in neurons. It plays a critical role in stress-dependent cardiac growth and has been implicated in immune regulation by controlling T-cell differentiation. It plays diverse roles in the control of cell growth, survival and apoptosis via p38 MAPK signalling in muscle-specific and/or growth factor-related transcription. It functions in the regulation of neuronal apoptosis. MEF2D features a conserved MADS-box (amino acids 2–57) and MEF2 domains (amino acids 58–86), which are critical for DNA binding and dimerization. It is vital for the differentiation and maintenance of skeletal, cardiac, and smooth muscle lineages. In the brain, MEF2 factors are key regulators of neuronal survival, synaptic plasticity, and synapse elimination. Dysregulation of MEF2D is linked to neurodegenerative diseases like Parkinson's, cardiac hypertrophy, and various cancers, including B-cell acute lymphoblastic leukemia (B-ALL) and rhabdomyosarcoma. This description may include information annotated by UniProt and/or Google AI.