Product Name:
MAFG-pS124
Product Number:
ab-pn617
Target Full Name: Transcription factor MafG
Target Alias: V-maf musculoaponeurotic fibrosarcoma oncogene homologue G
Product Type Specific: MAFG phosphosite-specific antibody
Antibody Code: PN617
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S124
Protein UniProt: O15525
Protein SigNET: MAFG
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human MAFG
Antibody Immunogen Sequence: TVAR(pS)PVAC
Antibody Immunogen Description: Corresponds to amino acid residues T120 to A127. S124 phosphorylation stabilizes protein. This is the major in vivo phosphorylation site in MAFG (≥10 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Rat | Mouse | Platypus | Chicken | Zebra fish.
Scientific Background: MAFG (v-maf avian musculoaponeurotic fibrosarcoma oncogene homolog G) is a small Maf family bZIP transcription factor (162 aa) that functions as a key regulator in cellular differentiation, particularly in erythropoiesis, and stress response. As a transcription factor, it forms heterodimers with NFE2L2 (NRF2) or P45 NFE2 to activate target genes or acts as a repressor when homodimerized, regulating antioxidant, detoxification, and metabolic processes. As part of the small Maf family (along with MafF and MafK), MAFG lacks a traditional transactivation domain. It contains a basic region and a leucine zipper (bZIP) motif that allows it to bind to DNA, specifically Maf Recognition Elements (MARE). As an activator, MAFG heterodimerizes with larger partners like NRF2 to regulate antioxidant responses. It is essential for activating alpha- and beta-globin gene expression in conjunction with P45 NFE2. As a transcriptional repressor, it forms homodimers (often requiring sumoylation at Lys-14). It represses genes involved in bile acid synthesis (e.g., Cyp8b1, Cyp7b1). It is subject to post-translational modifications, including acetylation (by CBP). MAFG activity is regulated by Sumoylation at the N-terminal region (required for repression activity) and phosphorylation at the C-terminal region (which stabilizes the protein). MAFG is widely expressed in various tissues but is notably high in the liver and lungs. Its expression is induced by oxidative stress (reactive oxygen species, electrophilic compounds) and heavy metals. It is implicated in cancer progression, with increased expression linked to poor prognosis in certain tumours and potential roles in neurodegenerative diseases. This description may include information annotated by UniProt and/or Google AI.