Product Name:
LEF1-pT155
Product Number:
ab-pn616
Target Full Name: Lymphoid enhancer-binding factor 1
Product Type Specific: LEF1 phosphosite-specific antibody
Antibody Code: PN616
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T155
Protein UniProt: Q9UJU2
Protein SigNET: LEF1
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human LEF1
Antibody Immunogen Sequence: VHPL(pT)PLIT(βA)C
Antibody Immunogen Description: Corresponds to amino acid residues V151 to T159. T155 phosphorylation regulates transcription, although one report indicates inhibition, wheras another describes induction. This is one of the major in vivo phosphorylation sites in LEF1 (≥11 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus). LEF1 is known to be phosphorylated at this site in vitro by NLK.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus macaque | Dog | Rat | Mouse | Frog | Zebra fish.
Scientific Background: LEF1 (Lymphoid enhancer-binding factor 1) is a nuclear transcription factor and functions as part of the TCF/LEF family of transcription factors. It is a key downstream mediator of the Wnt/beta-catenin signaling pathway, It functions in regulating cell proliferation, differentiation, and stem cell maintenance. LEF1 binds to beta-catenin, transforming it into a transcriptional activator of Wnt target genes (e.g., MYC, CCND1). PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and beta-catenin. It regulates T-cell receptor alpha enhancer function. It may acts as a positive regulator of odontoblast differentiation during mesenchymal tooth germ formation, expression is repressed during the bell stage by MSX1-mediated inhibition of beta-catenin signaling. It contains an HMG-box domain for DNA binding and an N-terminal domain for beta-catenin interaction. Some isoforms (Isoform 3) can act as transcriptional repressors by binding to co-repressors like CtBP when Wnt signaling is absent. It is primarily expressed in pre-B and T cells (lymphoid tissues) in thymus, but also in developing tissues like hair follicles and teeth. High expression of LEF1 is a marker for Chronic Lymphocytic Leukemia (CLL) and is associated with poor prognosis in various solid tumours (e.g., pancreatic, colon, esophageal, lung). It promotes epithelial-mesenchymal transition (EMT), aiding tumour invasion. This description may include information annotated by UniProt and/or Google AI.