Product Name:
Jun-pS243
Product Number:
ab-pn614
Target Full Name: Jun proto-oncogene-encoded AP1 transcription factor
Target Alias: Activator protein 1; AH119; AP1; AP-1; c-Jun; Jun A; Jun oncogene; P39; Proto-oncogene c-jun; RJG-9; V-jun avian sarcoma virus 17 oncogene homolog
Product Type Specific: Jun phosphosite-specific antibody
Antibody Code: PN614
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S243
Protein UniProt: P05412
Protein SigNET: Jun
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human Jun
Antibody Immunogen Sequence: PPL(pS)PIDM(βA)C
Antibody Immunogen Description: Corresponds to amino acid residues P240 to M247. S243 phosphorylation inhibits transcriptional activity by reducing binding of DNA and SP1. Its phosphorylation has been separately reported to both induce and inhibit FBXW7 interaction. This is one of the major in vivo phosphorylation sites in c-Jun (≥34 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus). Jun is known to be phosphorylated at this site in vitro by CDK1 (CDC2), CK2a1 (CSNK2A1), DYRK2, ERK1 (MAPK3), ERK2 (MAPK1), GSK3b, and mTOR (FRAP1).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus macaque | Dog | Rat | Mouse | Chicken | Frog | Zebra fish | Sea urchin.
Related Product 1: Jun S63 phosphorylation antibody (Cat. No.: AB-PN557)
Related Product 2: Jun S73 phosphorylation antibody (Cat. No.: AB-PN612)
Related Product 3: Jun T239 phosphorylation antibody (Cat. No.: AB-PN613)
Scientific Background: c-Jun is a transcription factor in the AP1 complex that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. It is activated by c-Jun N-terminal kinases (JNKs) through phosphorylation at specific residues (Ser-63 and Ser-73). It regulates gene expression (e.g., cyclin D1), cell proliferation, and apoptosis, playing a critical role in tissue development and neuronal responses to injury. It is activated by JNK phosphorylation. It promotes activity of NR5A1 when phosphorylated by HIPK3, which leads to increased steroidogenic gene expression upon cAMP signalling pathway stimulation. It features a bZIP domain (basic leucine zipper) crucial for DNA binding and dimerization. c-Jun is the cellular homologue of the viral oncoprotein v-Jun and is often upregulated in various cancers, promoting tumorigenesis. It is involved in activated KRas-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells. c-Jun is essential for normal mouse development and is often found overexpressed in damaged neurons and cancer cells. This description may include information annotated by UniProt and/or Google AI.