Product Name:
HSF1-pS303+pS307
Product Number:
ab-pn609
Target Full Name: Heat shock factor protein 1
Target Alias: Heat shock factor 1; Heat shock transcription factor 1; HSF 1; hsf1; HSF1_HUMAN; HSTF 1; HSTF1
Product Type Specific: HSF1 phosphosite-specific antibody
Antibody Code: PN609
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S303+S307
Protein UniProt: Q00613
Protein SigNET: HSF1
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human HSF1
Antibody Immunogen Sequence: EPP(pS)PPQ(pS)PRV(βA)C
Antibody Immunogen Description: Corresponds to amino acid residues E300 to V310. S303 and S307 phosphorylation induces a repression of transcriptional activity, and induce interaction with 14-3-3 epsilon and SUMO1. These are two of the major in vivo phosphorylation sites in HSF1 (≥59 and ≥55 reports, respectively, from high throughput mass spectrometry studies recorded in PhosphoSitePlus). HSF1 is known to be phosphorylated in vitro at S303: by BARK2 (GRK3, ADRBK2), ERK1 (MAPK3), ERK2 (MAPK1), GSK3b, p38a MAPK, p38b MAPK, p38d MAPK, and p38g MAPK, and at S307 by ERK1 (MAPK3), ERK2 (MAPK1), p38a MAPK (MAPK14), p38b MAPK (MAPK11), p38d MAPK (MAPK13), and p38g MAPK (MAPK12, ERK6).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Dog | Rat | Mouse | Platypus.
Related Product 1: HSF1 expression antibody (Cat. No.: AB-NN268-1)
Related Product 2: HSF1 expression antibody (Cat. No.: AB-NN268-2)
Related Product 3: HSF1 expression antibody (Cat. No.: AB-NN268-3)
Related Product 4: HSF1 expression antibody (Cat. No.: AB-NN268-4)
Scientific Background: HSF1, or heat shock factor 1, belongs to a family of Heat Shock transcription factors that activate the transcription of genes encoding products required for protein folding, processing, targeting, degradation, and function (2). The up-regulation of HSP (heat shock proteins) expression by stressors is achieved at the level of transcription through a heat shock element (HSE) and a transcription factor (HSF) (3, 4, 5). Most HSFs have highly conserved amino acid sequences. On all HSFs there is a DNA binding domain at the N-terminus. Hydrophobic repeats located adjacent to this binding domain are essential for the formation of active trimers. Towards the C-terminal region another short hydrophobic repeat exists, and is thought to be necessary for suppression of trimerization (6). There are two main heat shock factors, 1 and 2. Mouse HSF1 exists as two isoforms, however in higher eukaryotes HSF1 is found in a diffuse cytoplasmic and nuclear distribution in un-stressed cells. Once exposed to a multitude of stressors, it localizes to discrete nuclear granules within seconds. As it recovers from stress, HSF1 dissipates from these granules to a diffuse nuceloplasmic distribution. HSF2 on the other hand is similar to mouse HSF1, as it exists as two isoforms, the alpha form being more transcriptionally active than the smaller beta form (7, 8). Various experiments have suggested that HFS2 may have roles in differentiation and development (9, 10, 11). HSF1 is often overexpressed or overactive in many human malignancies, supporting cancer cell survival, proliferation, and metastasis by coordinating a unique transcriptional program distinct from the classical heat shock response. This description may include information annotated by UniProt and/or Google AI.