Product Name:
HNF4A-pS167
Product Number:
ab-pn608
Target Full Name: Hepatocyte nuclear factor 4-alpha
Target Alias: HNF4; HNF-4-alpha; NR2A1; TCF14; Transcription factor 14
Product Type Specific: HNF4A phosphosite-specific antibody
Antibody Code: PN608
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S167
Protein UniProt: P41235
Protein SigNET: HNF4A
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human HNF4A
Antibody Immunogen Sequence: RQIT(pS)PVSGC
Antibody Immunogen Description: Corresponds to amino acid residues R163 to G171. S167 phosphorylation induces transcription and interaction with PC4. This is one of the major in vivo phosphorylation sites in HNF4A (≥31 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus). HNF4A is known to be phosphorylated at this site in vitro by p38a MAPK (MAPK14), and p38b MAPK (MAPK11).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Dog | Rat | Mouse | Chicken | Frog .
Scientific Background: HNF4A (Hepatocyte Nuclear Factor 4 Alpha) is a transcription factor and member of the nuclear receptor superfamily. It functions as a homodimer to bind DNA and control the expression of genes involved in gluconeogenesis, lipid metabolism, and liver architecture. It controls the expression of hepatic genes during the transition of endodermal cells to hepatic progenitor cells during liver development, hepatocyte differentiation, and insulin production. It is also important for developing and maintaining the intestines, and nephron tubules. It likely activates the transcription of CYP2C38, and represses the CLOCK-BMAL1 transcriptional activity, and is essential for circadian rhythm maintenance and period regulation in the liver and colon cells. It features N-terminal activation (AF-1), DNA-binding (DBD), and ligand-binding (LBD) domains. It binds fatty acids, such as linoleic acid, as potential ligands. Phosphorylation (e.g., via ERK1) can inhibit its activity. Mutations in HNF4A cause MODY1, a type of inherited diabetes characterized by decreased insulin production that typically manifests in adolescence or early adulthood. Other HNF4A mutations can lead to high insulin levels (hyperinsulinism) in newborns and infants, sometimes causing severe low blood sugar. HNF4A not only influences metabolic diseases, but also playing roles in certain cancers and inflammatory conditions, such as ulcerative colitis. It is highly expressed in liver, pancreas, intestines, and kidneys. This description may include information annotated by UniProt and/or Google AI.