Product Name:
HCFC1-pS1507
Product Number:
ab-pn604
Target Full Name: Host cell factor 1
Target Alias: C1 factor; CFF; HCF; HCF1; HCF-1; HFC1; Host cell factor; Host cell factor C1 (VP16-accessory protein); MGC70925; VCAF; VP16 accessory
Product Type Specific: HCFC1 phosphosite-specific antibody
Antibody Code: PN604
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S1507
Protein UniProt: P51610
Protein SigNET: HCFC1
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human HCFC1
Antibody Immunogen Sequence: ELQV(pS)PGPR(βA)C
Antibody Immunogen Description: Corresponds to amino acid residues E1503 to R1511. The effect of S1507 phosphorylation is unclear. This is one of the major in vivo phosphorylation sites in HCFC1 (≥54 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus). HCFC1 is known to be phosphorylated at this site in vitro by ERK1 (MAPK3).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Dog | Mouse.
Scientific Background: HCFC1 (Host Cell Factor C1) is a critical transcriptional co-regulator protein. It acts as a molecular scaffold, binding to transcription factors (like THAP11, ZNF143) and chromatin-modifying complexes (like SET1/MLL) to regulate gene expression, cell cycle progression, and vitamin B12 (cobalamin) metabolism. It is a component of a THAP1/THAP3-HCFC1-OGT complex that is required for the regulation of the transcriptional activity of RRM1. It is also a coactivator for EGR2 and GABP2. It is a essential regulator of gene expression, facilitating the G1 to S phase transition in the cell cycle and promoting histone H3 'Lys-4' methylation. The protein features five N-terminal Kelch repeats and six HCF repeats, undergoing unique proteolytic maturation into noncovalently bound N- and C-terminal chains. HCFC1 gene mutations can lead to X-linked intellectual disability and complex metabolic disorders (cblX type), as it regulates the MMACHC gene, which is essential for vitamin B12 processing. Variants in the HCFC1 gene are also associated with other neurological problems, including epilepsy and severe developmental delay. HCFC1 was originally identified for its role in enabling Herpes Simplex Virus (HSV) infection. HCFC1 forms a multiprotein-DNA complex with the viral transactivator protein VP16 and POU2F1, and enables the transcription of the HSV viral immediate early genes. This description may include information annotated by UniProt and/or Google AI.