Product Name:
GTF2I-pS412
Product Number:
ab-pn602
Target Full Name: General transcription factor II-I
Target Alias: BAP135; BAP-135; Bruton tyrosine kinase-associated protein-135; BTKAP1; BTK-associated protein 135; BTK-associated protein-135; DIWS; DIWS1T; General II-I; General transcription factor IIi; GTFI; GTFII-I; IB291; SPIN; SRF-Phox1 interacting protein; WBSCR6; Williams-Beuren syndrome chromosome region 6 protein
Product Type Specific: GTF2I phosphosite-specific antibody
Antibody Code: PN602
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S412
Protein UniProt: P78347
Protein SigNET: GTF2I
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human GTF2I
Antibody Immunogen Sequence: FRRP(pS)TYGC
Antibody Immunogen Description: Corresponds to amino acid residues F408 to G415. S412 phosphorylation induces transcription. This is one of the major in vivo phosphorylation sites in GTF2I (≥147 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus). GTF2I is known to be phosphorylated at this site in vitro by PKG1.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Dog | Rat | Mouse | Platypus.
Related Product 1: GTF2F1 S385+T389 phosphorylation antibody (Cat. No.: AB-PK651)
Scientific Background: GTF2I (General transcription factor II-I) is a ubiquitous transcription factor that belongs to the TFII-I family. It interacts with the basal transcription machinery by coordinating the formation of a multiprotein complex at the c-Fos promoter, and linking specific signal responsive activator complexes. It features multiple repeats (typically six) that facilitate DNA binding and protein-protein interactions, including with transcription factors like Myc and signal transduction molecules like BTK (Bruton's tyrosine kinase). It promotes the formation of stable high-order complexes of SRF and PHOX1 and interacts cooperatively with PHOX1 to facilitate serum-inducible transcription of a reporter gene deriven by the c-Fos serum response element (SRE). It functions as a coregulator for USF1 by binding independently two promoter elements, a pyrimidine-rich initiator (Inr) and an upstream E-box. It is required for the formation of functional ARID3A DNA-binding complexes and for activation of immunoglobulin heavy-chain transcription upon B-lymphocyte activation. Isoform 1 is strongly expressed in fetal brain, weakly in adult brain, muscle, and lymphoblasts, and it is almost undetectable in other adult tissues. Other GTF2I isoforms are commonly expressed in all adult tissues. GTF2I is inked to cognitive, social, and anxiety-related behaviours; its deletion in mice impacts myelination, cortical thickness, and brain development. Hemizygosity (loss of one copy) of the GTF2I gene in the 7q11.23 region is strongly linked to the neurocognitive and behavioral phenotypes of Williams-Beuren syndrome. Increased copy number or altered expression of GTF2I is associated with autism-related traits. It is involved in B-cell activation and signal transduction. GTF2I mutations are frequently found in thymic epithelial tumours and some T-cell lymphomas. High levels are associated with tumour cell survival, while loss of the protein can lead to increased neuronal apoptosis. This description may include information annotated by UniProt and/or Google AI.