Product Name:
GATAD2B-pT120+pS122
Product Number:
ab-pn600
Target Full Name: Transcriptional repressor p66-beta
Target Alias: GATA zinc finger domain containing 2B; KIAA1150; P66 beta; P66/p68; P66beta; Transcription repressor p66 beta component of the MeCP1 complex; Transcriptional repressor p66 beta
Product Type Specific: GATAD2B phosphosite-specific antibody
Antibody Code: PN600
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T120+S122
Protein UniProt: Q8WXI9
Protein SigNET: GATAD2B
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human GATAD2B
Antibody Immunogen Sequence: CGRL(pT)P(pS)PDI
Antibody Immunogen Description: Corresponds to amino acid residues C117 to I125. The effect of T120 and S122 phosphorylation are unclear. These are two of the major in vivo phosphorylation sites in GATAD2B (≥90 and ≥86 reports, respectively, from high throughput mass spectrometry studies recorded in PhosphoSitePlus). GATAD2B is known to be phosphorylated in vitro at S122 by PRP4 (PRPF4B).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus macaque | Dog | Rat | Mouse | Zebra fish.
Scientific Background: GATAD2B (Transcriptional repressor p66-beta) is a transcriptional repressor that is widely expressed and plays a role in synapse development. It plays a crucial role in neurodevelopment by modulating gene expression and chromatin structure. It enhances MBD2-mediated repression, in combination with GATAD2A. It targets MBD3 to discrete loci in the nucleus, and it specifically by assisting in the binding of MBD2/3 to specific methylated DNA sites. It acts as a component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin. GATAD2B is highly expressed during embryonic development, acting as a crucial regulator in neurodevelopment and cortical patterning. Mutations or loss-of-function in GATAD2B cause GAND Syndrome (GATAD2B-associated neurodevelopmental disorder), characterized by intellectual disability, infantile hypotonia, apraxia of speech, epilepsy, and characteristic facial features. This description may include information annotated by UniProt and/or Google AI.