Product Name:
FOXM1-pT611
Product Number:
ab-pn592
Target Full Name: Forkhead box protein M1
Target Alias: FKH16; FKHL16; Forkhead box M1; Forkhead homolog 16; Forkhead-related protein FKHL16; FXM1; Hepatocyte nuclear factor 3 forkhead homolog 11; HFH11; HFH-11; HNF-3; HNF-3/fork-head homolog-11; INS-1; M-phase phosphoprotein 2; MPHOSPH2; MPM-2 reactive phosphoprotein 2; MPP2; TGT3; Transcription factor Trident; Trident; WIN; Winged helix factor from INS-1 cells; Winged-helix factor from INS-1 cells; Winged-helix transcription factor Trident
Product Type Specific: FOXM1 phosphosite-specific antibody
Antibody Code: PN592
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T611
Protein UniProt: Q08050
Protein SigNET: FOXM1
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human FOXM1
Antibody Immunogen Sequence: PISS(pT)PSK(βA)C
Antibody Immunogen Description: Corresponds to amino acid residues P607 to K614. T611 phosphorylation facilitates binding histone acetyltransferase CREB binding protein (CBP) to the FoxM1 transcriptional activation domain. This is one of the minor in vivo phosphorylation sites in FOXM1 based on ≥6 high throughput mass spectrometry reports recorded in PhosphoSitePlus. FOXM1 is known to be phosphorylated at this site in vitro by CDK1 (CDC2), CDK2, CDK4, and CDK6.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Dog | Rat | Mouse | Chicken | Frog.
Related Product 1: FOXK1 S441+S445 phosphorylation antibody (Cat. No.: AB-PN590)
Related Product 2: FOXK2 S424+S428 phosphorylation antibody (Cat. No.: AB-PN591)
Related Product 3: FOXO1 S256 phosphorylation antibody (Cat. No.: AB-PN593)
Related Product 4: FOXO1 S319 phosphorylation antibody (Cat. No.: AB-PN594)
Related Product 5: FOXO1 S329 phosphorylation antibody (Cat. No.: AB-PN595)
Related Product 6: FOXO3 T32 phosphorylation antibody (Cat. No.: AB-PN596)
Related Product 7: FOXO3 S294 phosphorylation antibody (Cat. No.: AB-PN597)
Related Product 8: FOXO3 S253 phosphorylation antibody (Cat. No.: AB-PN821)
Scientific Background: FOXM1 (Forkhead box protein M1) is a transcriptional factor in the Forkhead family. It features an N-terminal Repressor Domain (NRD), a conserved Forkhead (winged-helix) DNA Binding Domain (DBD), and a C-terminal Transactivation Domain (TAD). It promotesthe expression of cell cycle genes essential for DNA replication and mitosis (especially the G1/S and G2/M transitions). FOXM1 drives mitosis by activating genes such as Cdc25B, Aurora B kinase, and cyclin B1. In addition to control of cell proliferation, it is also involved in repair of DNA breaks participating in the DNA damage checkpoint response. It also promotes transcription of PHB2. It is essential for tissue development and regeneration but, when overexpressed (in up to 85% of certain cancers), acts as an oncogene in most human cancers. It is expressed in thymus, testis, small intestine, colon followed by ovary. It appears to be expressed only in adult organs containing proliferating/cycling cells or in response to growth factors. It is also expressed in epithelial cell lines derived from tumours, but not in resting cells. Isoform 2 is highly expressed in testis. This description may include information annotated by UniProt and/or Google AI.