Product Name:
Fos-pS362+pS363
Product Number:
ab-pn589
Target Full Name: Fos-c FBJ murine osteosarcoma oncoprotein-related transcription factor
Target Alias: Cellular oncogene fos
Product Type Specific: Fos phosphosite-specific antibody
Antibody Code: PN589
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S362+S363
Protein UniProt: P01100
Protein SigNET: Fos
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human Fos
Antibody Immunogen Sequence: CAHRKG(pS)(pS)SNE
Antibody Immunogen Description: Corresponds to amino acid residues A357 to E366. S362 and S363 phosphorylation stimulates transcriptional activity. These are two of the minor in vivo phosphorylation sites in Fos based on ≥4 and ≥1 high throughput mass spectrometry reports recorded in PhosphoSitePlus. Fos is known to be phosphorylated in vitro at S362 by RSK1 (RPS6KA1), and RSK2 (RPS6KA3).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus Macaque | Dog | Rat | Mouse | Platypus | Chicken | Frog.
Related Product 1: Fos T232 phosphorylation antibody (Cat. No.: AB-PN588)
Scientific Background: Fos is a transcription factor that belongs to the bZIP family, Fos subfamily. The Fos family of transcription factors (including c-Fos, FosB, FosL1, FosL2) are immediate-early genes encoding leucine zipper proteins that dimerize with Jun family proteins to form the Activator Protein-1 (AP-1) complex. In the heterodimer, FOS and JUN/AP-1 basic regions each seems to interact with symmetrical DNA half sites. with TGF-beta activation, it forms a multimeric SMAD3/SMAD4/JUN/FOS complex at the AP1/SMAD-binding site to regulate TGF-beta-mediated signaling. It has a critical function in regulating the development of cells destined to form and maintain the skeleton. It plays important roles in processes like cell growth, differentiation, and neuronal plasticity, with high expression levels often associated with neural stimulation and development. In growing cells, activates phospholipid synthesis, possibly by activating CDS1 and PI4K2A. This activity requires tyrosine-dephosphorylation and association with the endoplasmic reticulum. The Fos family of transcription factors (including c-Fos, FosB, FosL1, FosL2) are immediate-early genes encoding leucine zipper proteins that dimerize with Jun family proteins to form the Activator Protein-1 (AP-1) complex. While critical for normal development, overexpression of Fos can lead to tumor development and metastasis. This description may include information annotated by UniProt and/or Google AI.