Product Name:
ESRRA-pS19+pS22
Product Number:
ab-pn585
Target Full Name: Steroid hormone receptor ERR1
Target Alias: ERR- alpha; ERRa; ERRalpha; ESRL1; Estrogen receptor-like 1; Estrogen-related receptor alpha; Estrogen-related receptor, alpha; NR3B1
Product Type Specific: ESRRA phosphosite-specific antibody
Antibody Code: PN585
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S19+S22
Protein UniProt: P11474
Protein SigNET: ESRRA
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human ESRRA
Antibody Immunogen Sequence: CAEPA(pS)PD(pS)PKG
Antibody Immunogen Description: Corresponds to amino acid residues A15 to G25. S19 and S22 phosphorylation represses transactivation activity in response to coactivator. Phosphorylation enhanced the sumoylation at Lys-14, and contributes to regulating GRIP-1-dependent activated transcriptional activation by ERRα1. These are two of the major in vivo phosphorylation sites in ESRRA (≥21 and ≥18 reports, respectively, from high throughput mass spectrometry studies recorded in PhosphoSitePlus).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus macaque | Rat | Mouse.
Scientific Background: ESRRA (ERR1) is a 45.5 kDa transcription factor of the nuclear hormone receptor family, NR3 subfamily. It is a master transcriptional regulator of cellular energy metabolism, mitochondrial biogenesis, and lipid metabolism. It is essential for fatty acid oxidation, mitochondrial function, and energy metabolism. Its deficiency results in impaired metabolism and reduced adaptation to stress. ESRRA binds to an ERR-alpha response element (ERRE) containing a single consensus half-site, 5'-TNAAGGTCA-3'. It targets the medium-chain acyl coenzyme A dehydrogenase (MCAD) response element NRRE-1, the MCAD promoter and C1 region of the lactoferrin gene promoter. It requires dimerization and the coactivator, PGC-1A, for full activity. The ERRalpha/PGC1alpha complex is a regulator of energy metabolism. It induces the expression of PERM1 in the skeletal muscle. It is frequently overexpressed in various cancers (e.g., breast, ovarian, gastric, gallbladder), where it supports tumor growth, metastasis, and drug resistance. It is also implicated in controlling autophagic flux and innate immune responses. This description may include information annotated by UniProt and/or Google AI.