Product Name:
ERF-pT526
Product Number:
ab-pn584
Target Full Name: ETS domain-containing transcription factor ERF
Target Alias: Ets2 repressor factor; ETS-domain transcription factor ERF; PE2; PE-2
Product Type Specific: ERF phosphosite-specific antibody
Antibody Code: PN584
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T526
Protein UniProt: P50548
Protein SigNET: ERF
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human ERF
Antibody Immunogen Sequence: CGGPL(pT)PRR
Antibody Immunogen Description: Corresponds to amino acid residues G522 to R529. T526 phosphorylation inhibits ERF transcriptional repression by inducing its export from the nucleus, and regulates cell cycle progression. Phosphorylation induces transcriptional activity. by inhibition of ERF repression. This is the major in vivo phosphorylation site in ERF (≥150 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus). ERF is known to be phosphorylated at this site in vitro by ERK1, and ERK2.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Rhesus macaque | Rat | Mouse.
Scientific Background: ERF (ETS2 repressor factor) is a ubiquitously expressed ETS-family transcription factor that functions primarily as a strong transcriptional repressor, regulating cellular proliferation, differentiation, and suppressing Ets-associated tumorigenesis. It acts as a downstream effector in the Ras/MAPK signaling pathway, where phosphorylation by ERK1/2 MAP kinases causes its nuclear export and inactivation. It is a potent transcriptional repressor that binds to the H1 element of the Ets2 promoter. It may regulate other genes involved in cellular proliferation. ERF is required for extraembryonic ectoderm differentiation, ectoplacental cone cavity closure, and chorioallantoic attachment. It may be important for regulating trophoblast stem cell differentiation. Its highest levels of expression are in testis, ovary, pancreas, and heart. Mutations in the human ERF gene are associated with diseases like Craniosynostosis 4 (CRS4). ERF suppresses ets-induced transformation, and Its repression activity is often decreased in cells transformed by activated Ha-ras and v-src. This description may include information annotated by UniProt and/or Google AI.