Product Name:
ERa-pS167
Product Number:
ab-pn583
Target Full Name: Estrogen receptor alpha
Target Alias: ER; Era; ESR; ESR1; ESTR; ESTRA; Estradiol receptor; estrogen receptor 1; NR3A1
Product Type Specific: ERa phosphosite-specific antibody
Antibody Code: PN583
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S167
Protein UniProt: P03372
Protein SigNET: ERa
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human ERa
Antibody Immunogen Sequence: ERLA(pS)TNDKGC
Antibody Immunogen Description: Corresponds to amino acid residues E163 to G172. S167 phosphorylation induces transcriptional activity and regulates cell growth, apoptosis, transcription, acetylation, activation and molecular association, including inducing interaction with SRC-3. This is one of the major in vivo phosphorylation sites in the estrogen receptor (≥5 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus). ERa is known to be phosphorylated at this site in vitro by Akt1 (PKBa), Akt2 (PKBb), AurKA, CK2a1 (CSNK2A1), IKKE, p70S6K (RPS6KB1), RSK1 (RPS6KA1), and RSK2 (RPS6KA3).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Dog | Mouse | Chicken | Frog.
Scientific Background: ERa (Estrogen receptor alpha; ER-alpha; ESR1) is a 67.5 kDa ligand-dependent transcription factor that belongs to the nuclear hormone receptor family, NR3 subfamily. It features an N-terminal modulator domain (AF-1), a central DNA-binding domain, and a C-terminal ligand-binding domain (AF-2). It binds estrogen to regulate gene expression in reproductive and metabolic tissues, acting as a crucial driver in over 70% of breast cancers. Structurally, it is a nuclear receptor with DNA-binding and ligand-binding domains that forms dimers to influence transcription and can function as an RNA-binding protein. It regulates eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. It controls metabolism, and sexual development, largely in reproductive tissues. Ligand binding induces a conformational change allowing subsequent or combinatorial association with multiprotein coactivator complexes through LXXLL motifs of their respective components. Ligand-dependent nuclear transactivation involves either direct homodimer binding to a palindromic estrogen response element (ERE) sequence or association with other DNA-binding transcription factors, such as AP-1/c-Jun, c-Fos, ATF-2, Sp1 and Sp3, to mediate ERE-independent signaling. Mutual transrepression occurs between the estrogen receptor (ER) and NF-kappa-B in a cell-type specific manner. Isoforms lacking one or several functional domains are thought to modulate transcriptional activity by competitive ligand or DNA binding and/or heterodimerization with the full-length receptor. It is essential for MTA1-mediated transcriptional regulation of BRCA1 and BCAS3. Isoform 3 is involved in activation of NOS3 and endothelial nitric oxide production. Isoform 3 can bind to ERE and inhibit isoform 1. Isoform 3 is not expressed in the pituitary gland. This description may include information annotated by UniProt and/or Google AI.