Product Name:
Elk1-pS324
Product Number:
ab-pn581
Target Full Name: ETS domain-containing protein Elk-1
Target Alias: ELK1; ELK1, member of ETS oncogene family; ETS-domain protein Elk-1; ETS-domain protein ELK-1
Product Type Specific: Elk1 phosphosite-specific antibody
Antibody Code: PN581
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S324
Protein UniProt: P19419
Protein SigNET: Elk1
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human Elk1
Antibody Immunogen Sequence: ELPL(pS)PSLLGC
Antibody Immunogen Description: Corresponds to amino acid residues E320 to G329. S324 phosphorylation stimulates transcriptional activity by MAD2L2 and potentiates ternary complex formation. This is the major in vivo phosphorylation site in Elk-1 (≥20 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus). Elk1 is known to be phosphorylated at this site in vitro by ERK1 (MAPK3), ERK2 (MAPK1), and JNK2 (MAPK9).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Dog | Rat | Mouse.
Scientific Background: Elk1 (ETS domain-containing protein Elk-1) is a transcription factor that belongs to the ETS family. It features an N-terminal ETS domain (DNA binding), a B-box (SRF binding), and a C-terminal activation domain (phosphorylation sites). Elk1 binds to purine-rich DNA sequences. It forms a ternary complex with SRF (serum response factor) and the ETS and SRF motifs of the serum response element (SRE) on the promoter region of immediate early genes such as FOS and IER2. It induces target gene transcription upon MAPK-signalling pathways stimulation. It is activated via phosphorylation by MAP kinases (ERK, JNK, p38) upon extracellular stimulation, allowing it to recruit co-activators like p300. It is silenced by SUMOylation under basal conditions and activated when those groups are removed, allowing phosphorylation. It is primarily found in the nucleus, but also found in the cytoplasm and neuronal processes. It is highly expressed in lung and testis. Misregulation of Elk-1 is linked to cancer growth (e.g., in KRAS-mutated NSCLC, MDPI) and neurodegenerative conditions. This description may include information annotated by UniProt and/or Google AI.