Product Name:
CREB1-pS129+pS133
Product Number:
ab-pn577
Target Full Name: cAMP-response element-binding protein 1
Target Alias: CAMP responsive element binding protein 1; CAMP-response element binding protein; CREB1; CREB-1
Product Type Specific: CREB1 phosphosite-specific antibody
Antibody Code: PN577
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S129+S133
Protein UniProt: P16220
Protein SigNET: CREB1
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human CREB1
Antibody Immunogen Sequence: EIL(pS)RRP(pS)YRK(βA)C
Antibody Immunogen Description: Corresponds to amino acid residues E126 to K136. S129 and S133 phosphorylation stimulate transcriptional activity and induce interactions with CBP, DNA, MEIS1, Myb, Nur77, RGS13, SOX9 and p300. S129 is a minor in vivo phosphorylation site in CREB1 (≥2 reports from high throughput mass spectrometry reports recorded in PhosphoSitePlus), but S133 is the major site (≥65 reports). CREB1 is known to be phosphorylated in vitro at S129 by BARK2 (GRK3, ADRBK2), GSK3a, and GSK3b, and at S133 by Akt1 (PKBa), Btk, CaMK1a, CaMK2a, CaMK4, DYRK3, MAPKAPK2, MSK1 (RPS6KA5), MSK2 (RPS6KA4), PKACa (PRKACA), PKCa (PRKCA), PKCe (PRKCE), PKD1 (PRKCM, PRKD1), RSK1 (RPS6KA1), RSK2 (RPS6KA3), RSK3 (RPS6KA2), SGK1, TSSK4, and VRK1.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rat | Mouse.
Related Product 1: CREB S133 phosphorylation antibody (Cat. No.: AB-PN553)
Scientific Background: CREB1 (cAMP-responsive element-binding protein 1) is a member of the cAMP responsive element binding protein family of transcription factors that are critical mediators of gene expression in response to extracellular signals and are essential regulators of adaptive behavior and long-term memory formation, symaptic plasticity, learning, and neuronal survival. It regulates gluconeogenesis and energy metabolism in the liver. It is also for cell differentiation, particularly in immune responses and melanocytes. CREB1 belongs to the bZIP (basic leucine zipper) family, forming homodimers or heterodimers with related proteins like CREM and ATF1. CREB1 binds to the cAMP-responsive element (CRE) (octameric palindrome (TGACGTCA) to induce gene transcription. CREB also binds to promoters containing activator protein 1 (AP-1) sites to alter target-gene expression. Activation of CREB1 by PKACa (PRKACA), mediated phosphorylation at Ser-133 has been implicated in the survival of mammalian cells. However, Ser-133 can also be phosphorylated in vitro Akt1 (PKBa), Btk, CaMK1a, CaMK2a, CaMK4, DYRK3, MAPKAPK2, MSK1 (RPS6KA5), MSK2 (RPS6KA4), PKCa (PRKCA), PKCe (PRKCE), PKD1 (PRKCM), RSK1 (RPS6KA2), RSK2 (RPS6KA3), RSK3 (RPS6KA1), SGK1, TSSK4, VRK1. Ser-142 and Ser-143 phosphorylation potentially inhibits transcription. Mice lacking CREB in the central nervous system during development show extensive apoptosis of postmitotic neurons. Dysfunction of CREB1 is associated with neurodegenerative diseases (e.g., Alzheimer's) and cognitive decline. CREB1 is also involved in cancer (e.g., melanomas, myeloid leukemias). This description may include information annotated by UniProt and/or Google AI.