Product Name:
CDC5L-pT385
Product Number:
ab-pn576
Target Full Name: Cell division cycle 5-like protein
Target Alias: CDC5; CDC5 cell division cycle 5-like; Cdc5-like protein; CDC5-related protein; CEF1; Cell division cycle 5, S. pombe, homolog; PCDC5RP; Pombe cdc5-related protein
Product Type Specific: CDC5L phosphosite-specific antibody
Antibody Code: PN576
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T385
Protein UniProt: Q99459
Protein SigNET: CDC5L
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human CDC5L
Antibody Immunogen Sequence: CGGLN(pT)PLHE
Antibody Immunogen Description: Corresponds to amino acid residues C381 to E389. The effect of T385 phosphorylation is unclear. This is one of the major in vivo phosphorylation sites in CDC5L (≥66 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus). CDC5L is known to be phosphorylated at this site in vitro by ERK1 (MAPK3).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus macaque | Dog | Rat | Mouse | Platypus | Chicken | Zebra fish | Honey bee | Red bread mold.
Scientific Background: CDC5L (Cell division cycle 5-like protein) is 92 kDa DNA-binding and transcription protein that belongs to the CEF1 family, and functions as a dimer. It acts as a core component of the human Prp19/CDC5L spliceosomal complex, which forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. The PRP19-CDC5L complex may also play a role in the response to DNA damage (DDR). It interacts with ATR (ataxia-telangiectasia and Rad3-related) and is required for the S-phase checkpoint. It is essential for pre-mRNA splicing (second catalytic step), cell cycle progression, and DNA damage repair. CDC5L is involved in cell cycle control and has a role in pre-mRNA splicing as core component of precatalytic, catalytic and postcatalytic spliceosomal complexes. It acts as a transcription activator and has been linked to the regulation of chondrocyte differentiation. It is ubiquitously expressed in both fetal and adult tissues, including lymphocytes, T-cells, and proliferating cells. Defective signalling of CDC5L is associated with intracerebral hemorrhage and polycystic kidney disease. It is overexpressed in osteosarcoma and cervical tumours. Its downregulation or silencing inhibits cell migration and triggers apoptosis in gastric cancer cells. This description may include information annotated by UniProt and/or Google AI.