Product Name:
AML2-pT231
Product Number:
ab-pn569
Target Full Name: Runt-related transcription factor 3
Target Alias: Acute myeloid leukemia 2 protein; CBFA3; CBF-alpha 3; Core-binding factor, alpha 3; Oncogene AML-2; PEA2-alpha C; PEBP2A3; PEBP2-alpha C; Polyomavirus enhancer binding protein 2 alpha C subunit; SL3/AKV core-binding factor alpha C; SL3-3 enhancer factor 1 alpha C
Product Type Specific: AML2 phosphosite-specific antibody
Antibody Code: PN569
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T231
Protein UniProt: Q13761
Protein SigNET: AML2
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human AML2
Antibody Immunogen Sequence: SQPQ(pT)PIQGC
Antibody Immunogen Description: Corresponds to amino acid residues S227 to G235. T231 phosphorylation inhibits activity and induces cell growth. This is a minor in vivo phosphorylation sites in AML2 (≥20 reports from high throughput mass spectrometry studies in PhosphoSitePlus).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Mouse | Chicken.
Related Product 1: AML1 T273 phosphorylation antibody (Cat. No.: AB-PN568)
Scientific Background: AML2 (Runt-related transcription factor-3) is a transcription factor that forms the heterodimeric complex core-binding factor (CBF) with CBFB. RUNX members modulate the transcription of their target genes through recognizing the core consensus binding sequence 5'-TGTGGT-3', or very rarely, 5'-TGCGGT-3', within their regulatory regions via their runt domain. CBFB is a non-DNA-binding regulatory subunit that allosterically enhances the sequence-specific DNA-binding capacity of RUNX. The heterodimers bind to the core site of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL3 and GM-CSF promoters. It may regulate cellular proliferation and/or differentiation. In association with ZFHX3, AML2 upregulates CDKN1A promoter activity following TGF-beta stimulation. CBF complexes repress ZBTB7B transcription factor during cytotoxic (CD8+) T cell development. They bind to RUNX-binding sequence within the ZBTB7B locus acting as transcriptional silencer and allowing for cytotoxic T cell differentiation. CBF complexes binding to the transcriptional silencer is essential for recruitment of nuclear protein complexes that catalyze epigenetic modifications to establish epigenetic ZBTB7B silencing. AML2 is expressed in gastric cancer tissues (at protein level). This description may include information annotated by UniProt and/or Google AI.