Product Name:
PCYT1A-pS329+pS331
Product Number:
ab-pn561
Target Full Name: CTP: phosphocholine cytidylyltransferase isoform A
Target Alias: CCT A; CCT-alpha; CCTA; Choline-phosphate cytidylyltransferase A; CT; CT A; CTA; CTP:phosphocholine cytidylyltransferase A; CTPCT; PCY1A; PCYT1; PCYT1A; phosphate cytidylyltransferase 1, choline, alpha; Phosphorylcholine transferase A
Product Type Specific: PCYT1A phosphosite-specific antibody
Antibody Code: PN561
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S329+S331
Protein UniProt: P49585
Protein SigNET: P49585
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human PCYT1A; CTPCT; CCTA sequence peptide
Antibody Immunogen Sequence: TRER(pS)P(pS)PSF(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues T325 to F334; Near the C-terminus of the protein. These are among the main in vivo phosphorylation sites in PCYT1A.
Production Method: Corresponds to amino acid residues T325 to F334; Near the C-terminus of the protein. These are among the main in vivo phosphorylation sites in PCYT1A.
Antibody Modification: PCYT1A phosphosite-specific antibody
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 40-45 kDa.
Related Product 1: PCYT1A-pT342+pS343 phosphosite-specific antibody (Cat. No.: AB-PN547)
Related Product 2: PCYT1A-pY359+pS362 phosphosite-specific antibody (Cat. No.: AB-PN548)
Related Product 3: PCYT1B-pS315+pS319 phosphosite-specific antibody (Cat. No.: AB-PN546)
Scientific Background: PCYT1A is the rate limiting enzyme in the biosynthetic pathway for phosphatidylcholine. It catalyzes the reaction: CTP + phosphocholine = diphosphate + CDP-choline. It is an ambiquitous enzyme that resides in the cytoplasm of cells in an inactive form. Diacylglycerol, acidic phospholipids and fatty acids drive the binding of PCYT1A to the endoplasmic reticulum and stimulation of its catalytic activity.
