Product Name:
SHIP1-pY187
Product Number:
ab-pn560
Target Full Name: Phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1
Target Alias: Inositol polyphosphate-5-phosphatase of 145 kDa; Inositol polyphosphate-5-phosphatase, 145kDa; INPP5D; P150Ship; Phosphatidylinositol-3,4,5-trisphosphate 5-phosphatase 1; SH2-containing inositol 5'-phosphatase 1; SHIP1; SHIP-1; SIP-145
Product Type Specific: SHIP1 phosphosite-specific antibody
Antibody Code: PN560
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y187
Protein UniProt: Q92835
Protein SigNET: SHIP1
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human SHIP1 (INPP5D) sequence peptide Cat. No.: PE-04ARJ90
Antibody Immunogen Sequence: CAIQD(pY)LSTQ(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues A183 to Q191; in the region between the SH2 and Exo-endo_phos domains. The effect of Y187 phosphorylation is unclear. This has not been confirmed as an in vivo phosphorylation site by mass spectrometry according to PhosphoSitePlus. This phosphorylation site is also conserved in chicken.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Rat | Mouse | Chicken.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 132-145 kDa.
Antibody Specificity: High-very high
Antibody Cross Reactivity: No significant cross-reactive proteins detected in phenylarsine oxide (PAO)+vanadate-treated HeLa cells, EGF-treated A431 cells and insulin-treated MCF7 cells, when these cells were homogenized in SDS-PAGE sample buffer, except for a 26K cross-reactive protein in EGF-treated HeLa cells.
Related Product 1: SHIP1-pY187 blocking peptide
Related Product 2: SHIP1-1 pan-specific antibody (Cat. No.: AB-NP044-1)
Related Product 3: SHIP1-2 pan-specific antibody (Cat. No.: AB-NP044-2)
Related Product 4: SHIP1-3 pan-specific antibody (Cat. No.: AB-NP044-3)
Scientific Background: SHIP1 (SH2 domain-containing inositol 5-phosphatase 1; INPP5D) is a phosphatidylinositol (PtdIns) phosphatase that specifically hydrolyzes the 5-phosphate of phosphatidylinositol-3,4,5-trisphosphate (PtdIns(3,4,5)P3) to produce PtdIns(3,4)P2, to negatively regulate PI3K (phosphoinositide 3-kinase) pathways. It acts as a negative regulator of B-cell antigen receptor signalling, and mediates signalling from the FC-gamma-RIIB receptor (FCGR2B) as well as a negative regulator of myeloid cell proliferation/survival and chemotaxis, neutrophil migration, mast cell degranulation, immune cells homeostasis, integrin alpha-IIb/beta-3 signalling in platelets and JNK signalling in B-cells. It regulates proliferation of osteoclast precursors, macrophage programming, phagocytosis and activation and is required for endotoxin tolerance. It may also hydrolyze PtdIns(1,3,4,5)P4. It interacts with adaptor proteins such as SHC, GABs, and DOKs. It features an N-terminal SH2 domain, a central catalytic 5-phosphatase domain, and a C-terminal proline-rich region. SHIP1 is primarily expressed in immune cells like B cells, mast cells, and myeloid cells. Primarily cytoplasmic but recruited to the plasma membrane upon receptor activation. It is expressed in microglial cells, where it regulates immune response, making it a key risk factor and therapeutic target for Alzheimer's disease. This description may include information annotated by UniProt and/or Google AI.