Product Name:

SHIP1-pY187


Product Number:

ab-pn560

Price:

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$89.00
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Target Full Name: Phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1

Target Alias: Inositol polyphosphate-5-phosphatase of 145 kDa; Inositol polyphosphate-5-phosphatase, 145kDa; INPP5D; P150Ship; Phosphatidylinositol-3,4,5-trisphosphate 5-phosphatase 1; SH2-containing inositol 5'-phosphatase 1; SHIP1; SHIP-1; SIP-145

Product Type Specific: SHIP1 (INPP5D) phosphosite-specific antibody

Antibody Code: PN560

Antibody Target Type: Phosphosite-specific

Antibody Phosphosite: Y187

Protein UniProt: Q92835

Protein SigNET: Q92835

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human SHIP1 (INPP5D) sequence peptide Cat. No.: PE-04ARJ90

Antibody Immunogen Sequence: CAIQD(pY)LSTQ(bA)C

Antibody Immunogen Description: Corresponds to amino acid residues A183 to Q191; In the region between the SH2 and Exo-endo_phos domains.

Production Method: Corresponds to amino acid residues A183 to Q191; In the region between the SH2 and Exo-endo_phos domains.

Antibody Modification: SHIP1 (INPP5D) phosphosite-specific antibody

Antibody Concentration: 1 mg/ml

Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Antibody microarray

Antibody Dilution Recommended: 2 µg/ml for immunoblotting

Antibody Species Reactivity: Human

Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 132-145 kDa.

Antibody Specificity: High-very high

Antibody Cross Reactivity: No significant cross-reactive proteins detected in phenylarsine oxide (PAO)+vanadate-treated HeLa cells, EGF-treated A431 cells and insulin-treated MCF7 cells, when these cells were homogenized in SDS-PAGE sample buffer, except for a 26K cross-reactive protein in EGF-treated HeLa cells.

Scientific Background: SHIP1 (INPP5D) is a phosphatidylinositol (PtdIns) phosphatase that specifically hydrolyzes the 5-phosphate of phosphatidylinositol-3,4,5-trisphosphate (PtdIns(3,4,5)P3) to produce PtdIns(3,4)P2, to negatively regulate PI3K (phosphoinositide 3-kinase) pathways. It acts as a negative regulator of B-cell antigen receptor signalling, and mediates signalling from the FC-gamma-RIIB receptor (FCGR2B) as well as a negative regulator of myeloid cell proliferation/survival and chemotaxis, neutrophil migration, mast cell degranulation, immune cells homeostasis, integrin alpha-IIb/beta-3 signalling in platelets and JNK signalling in B-cells. It regulates proliferation of osteoclast precursors, macrophage programming, phagocytosis and activation and is required for endotoxin tolerance. It may also hydrolyze PtdIns(1,3,4,5)P4.