Product Name:
RPS6-pS235+pS236+pS240
Product Number:
ab-pn559
Target Full Name: 40S Ribosomal S6
Target Alias: Phosphoprotein NP33; Ribosomal protein S6; RS6; S6 Ribosomal Protein
Product Type Specific: Ribosomal proteinS6 phosphosite-specific antibody
Antibody Code: PN559
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S235+S236+S240
Protein UniProt: P62753
Protein SigNET: P62753
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human RPS6 sequence peptide Cat. No.: PE-04AQR95
Antibody Immunogen Sequence: RRL(pS)(pS)LRA(pS)TS(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues R232 to S242; In the C-terminus of the ribosomal protein. These are the major in vivo phosphorylation sites in the S6 protein.
Production Method: Corresponds to amino acid residues R232 to S242; In the C-terminus of the ribosomal protein. These are the major in vivo phosphorylation sites in the S6 protein.
Antibody Modification: Ribosomal proteinS6 phosphosite-specific antibody
Antibody Concentration: 0.1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Medium immunoreactivity of a target-sized protein by Western blotting in MCF7 + insulin (homogenized in SDS-PAGE buffer).
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 28-34 kDa.
Antibody Specificity: Very high
Antibody Cross Reactivity: No significant cross-reactive proteins detected in phenylarsine oxide (PAO)+vanadate-treated HeLa cells, EGF-treated A431 cells and insulin-treated MCF7 cells, when these cells were homogenized in SDS-PAGE sample buffer.
Related Product 1: RPS6-pS235+pS236+pS240 blocking peptide
Related Product 2: RPS6 (230-238) KinSub - 40S ribosomal protein S6 (K230-R238, human) peptide; p70S6K protein kinase substrate peptide
Related Product 3: RPS6 (230-239) KinSub - 40S ribosomal protein S6 (K230-A239, human) peptide; RSK protein kinase substrate peptide
Scientific Background: RPS6 plays an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA that are important in protein synthesis. It belongs to the ribosomal protein S6e family. Growth factor stimulation of cultured cells leads to stong phosphorylation of RPS6 at multiple sites located in its C-terminus, which enhances it translational activity. This phosphorylation may be mediated by several protein kinases including those in the p70 S6 kinase and RSK families.
