Product Name:
Jun-pS63
Product Number:
ab-pn557
Target Full Name: Jun proto-oncogene-encoded AP1 transcription factor
Target Alias: Activator protein 1; AH119; AP1; AP-1; c-Jun; Jun A; Jun oncogene; P39; Proto-oncogene c-jun; RJG-9; V-jun avian sarcoma virus 17 oncogene homolog
Product Type Specific: Jun phosphosite-specific antibody
Antibody Code: PN557
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S63
Protein UniProt: P05412
Protein SigNET: Jun
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human Jun sequence peptide Cat. No.: PE-04ARC85
Antibody Immunogen Sequence: DLLT(pS)PDVGC
Antibody Immunogen Description: Corresponds to amino acid residues D59 to G67; in the N-terminus of the transcription factor. S63 phosphorylation stimulates transcriptional activity and binding to PIN1, although it has been reported to decrease binding to DNA and JNK1. This is one of the major in vivo sites of phosphorylation of c-Jun (≥44 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus). Jun is known to be phosphorylated at this site in vitro by CDK1 (CDC2), CDK3, CK2a1 (CSNK2A1), ERK1 (MAPK3), ERK2 (MAPK1), ERK7 (MAPK15), JNK1 (MAPK8), JNK2 (MAPK9), JNK3 (MAPK10), PBK (TOPK), PKD1 (PRKCM, PRKD1), Plk3 (CNK), and VRK1.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Rhesus Macaque | Dog | Rat | Mouse | Chicken | Frog | Zebra fish.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 40-45 kDa.
Antibody Specificity: Very high
Antibody Cross Reactivity: No significant cross-reactive proteins detected in phenylarsine oxide (PAO)+vanadate-treated HeLa cells, EGF-treated A431 cells and insulin-treated MCF7 cells, when these cells were homogenized in SDS-PAGE sample buffer.
Related Product 1: Jun (59-67) pS63 antibody blocking peptide (Cat. No.: PE-04ARC85)
Related Product 2: Jun S73 phosphorylation antibody (Cat. No.: AB-PN612)
Related Product 3: Jun T239 phosphorylation antibody (Cat. No.: AB-PN613)
Scientific Background: c-Jun is a transcription factor in the AP1 complex that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. It is activated by c-Jun N-terminal kinases (JNKs) through phosphorylation at specific residues (Ser-63 and Ser-73). It regulates gene expression (e.g., cyclin D1), cell proliferation, and apoptosis, playing a critical role in tissue development and neuronal responses to injury. It is activated by JNK phosphorylation. It promotes activity of NR5A1 when phosphorylated by HIPK3, which leads to increased steroidogenic gene expression upon cAMP signalling pathway stimulation. It features a bZIP domain (basic leucine zipper) crucial for DNA binding and dimerization. c-Jun is the cellular homologue of the viral oncoprotein v-Jun and is often upregulated in various cancers, promoting tumorigenesis. It is involved in activated KRas-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells. c-Jun is essential for normal mouse development and is often found overexpressed in damaged neurons and cancer cells. This description may include information annotated by UniProt and/or Google AI.