Product Name:
Jun-pS63
Product Number:
ab-pn557
Target Full Name: Jun transcription factor (AP1)
Target Alias: Activator protein 1; AH119; AP1; AP-1; c-Jun; Jun A; Jun oncogene; P39; Proto-oncogene c-jun; RJG-9; V-jun avian sarcoma virus 17 oncogene homolog
Product Type Specific: Jun phosphosite-specific antibody
Antibody Code: PN557
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S63
Protein UniProt: P05412
Protein SigNET: P05412
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human Jun sequence peptide Cat. No.: PE-04ARC85
Antibody Immunogen Sequence: DLLT(pS)PDVGC
Antibody Immunogen Description: Corresponds to amino acid residues D59 to G67; In the N-terminus of the transcription factor. These are the major in vivo sites of phosphorylation of c-Jun.
Production Method: Corresponds to amino acid residues D59 to G67; In the N-terminus of the transcription factor. These are the major in vivo sites of phosphorylation of c-Jun.
Antibody Modification: Jun phosphosite-specific antibody
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 40-45 kDa.
Antibody Specificity: Very high
Antibody Cross Reactivity: No significant cross-reactive proteins detected in phenylarsine oxide (PAO)+vanadate-treated HeLa cells, EGF-treated A431 cells and insulin-treated MCF7 cells, when these cells were homogenized in SDS-PAGE sample buffer.
Related Product 1: Jun-pS63 blocking peptide
Scientific Background: c-Jun is a ranscription factor in the AP1 complex that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. It promotes activity of NR5A1 when phosphorylated by HIPK3, which leads to increased steroidogenic gene expression upon cAMP signalling pathway stimulation. It is involved in activated KRas-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells.
