Product Name:
EEF2-pT57
Product Number:
ab-pn555
Target Full Name: Eukaryotic elongation factor 2
Target Alias: EEF-2; EF-2; EF2; Elongation factor 2; eukaryotic translation elongation factor 2; polypeptidyl-tRNA translocase
Product Type Specific: EEF2 phosphosite-specific antibody
Antibody Code: PN555
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T57
Protein UniProt: P13639
Protein SigNET: P13639
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human EEF2 sequence peptide Cat. No.: PE-04AQY90
Antibody Immunogen Sequence: ETRF(pT)DTR(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues E53 to R60; In the N-terminal portion of the protein. One of the major in vivo phosphorylation sites in EEF2.
Production Method: Corresponds to amino acid residues E53 to R60; In the N-terminal portion of the protein. One of the major in vivo phosphorylation sites in EEF2.
Antibody Modification: EEF2 phosphosite-specific antibody
Antibody Concentration: 0.5 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Strong immunoreactivity of a target-sized protein by Western blotting in A431 and MCF7 cells.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 95-105 kDa.
Antibody Specificity: Very high
Antibody Cross Reactivity: No significant cross-reactive proteins detected in A431, HeLa and MCF7 cells.
Related Product 1: EEF2-pT57 blocking peptide
Scientific Background: EEF2 catalyzes the GTP-dependent ribosomal translocation step during translation elongation in protein synthesis. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. It catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.
