Product Name:
ATF2-pT69 + pT71
Product Number:
ab-pn552
Target Full Name: Transcription factor ATF2
Target Alias: Activating 2; Activating transcription factor 2; CAMP response element binding protein CRE- BP1; CREB2; CREBP1; CRE-BP1; Cyclic-AMP-dependent ATF-2; HB16; MXBP protein; TREB7
Product Type Specific: ATF2 phosphosite-specific antibody
Antibody Code: PN552
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T69+T71
Protein UniProt: P15336
Protein SigNET: P15336
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human ATF2 sequence peptide Cat. No.: PE-04ARD99
Antibody Immunogen Sequence: ADQ(pT)P(pT)PTR(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues A66 to R74; In the N-terminus of the transcription factor. These are the major in vivo sites of phosphorylation of ATF2.
Production Method: Corresponds to amino acid residues A66 to R74; In the N-terminus of the transcription factor. These are the major in vivo sites of phosphorylation of ATF2.
Antibody Modification: ATF2 phosphosite-specific antibody
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 55-70 kDa.
Antibody Specificity: High-very high
Antibody Cross Reactivity: Weak 37 and 43 kDa cross-reactive proteins were detected in phenylarsine oxide (PAO)+vanadate-treated HeLa cells, EGF-treated A431 cells, and insulin-treated MCF7 cells, when these cells were directly homogenized into SDS-PAGE sample buffer.
Related Product 1: ATF2-pT69 + pT71 blocking peptide
Scientific Background: ATF2 is a transcriptional activator which regulates the transcription of various genes, including those involved in anti-apoptosis, cell growth, and DNA damage response. In combination with its binding partner, ATF2 binds to CRE (cAMP response element) consensus sequences (5'-TGACGTCA-3') or to AP-1 (activator protein 1) consensus sequences (5'-TGACTCA-3'). In the nucleus, it contributes to global transcription and the DNA damage response, in addition to specific transcriptional activities that are related to cell development, proliferation and death. It exhibits histone acetyltransferase (HAT) activity that specifically acetylates histones H2B and H4. In concert with CUL3 and RBX1, it promotes the degradation of KAT5 thereby attenuating its ability to acetylate and activate ATM.
