Product Name:
PRMT5-pT634
Product Number:
ab-pn549
Target Full Name: Protein arginine N-methyltransferase 5
Target Alias: 72 kDa ICln-binding protein; ANM5; Histone-arginine N-methyltransferase PRMT5; HMT1 hnRNP methyltransferase-like 5; HRMT1L5; IBP72; Jak-binding protein 1; JBP1; PRMT5; protein arginine methyltransferase 5; Protein arginine N-methyltransferase 5; Shk1 kinase-binding protein 1 homolog; SKB1; SKB1 homolog; SKB1Hs
Product Type Specific: PRMT5 phosphosite-specific antibody
Antibody Code: PN549
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T634
Protein UniProt: O14744
Protein SigNET: PRMT5
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human PRMT5 sequence peptide Cat. No.: PE-04ARI90
Antibody Immunogen Sequence: GRSY(pT)IGL(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues G630 to L637; At the C-terminus of the protein. The effect of T634 phosphorylation is unclear. This phosphosite located at the C-terminus of PRMT5 has not been identified as phosphorylated in vivo based on high throughput mass spectrometry reports recorded in PhosphoSitePlus.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Rat | Mouse.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 70-75 kDa.
Antibody Specificity: Very high
Antibody Cross Reactivity: No significant cross-reactive proteins detected in phenylarsine oxide (PAO)+vanadate-treated HeLa cells, EGF-treated A431 cells and insulin-treated MCF7 cells, when these cells were homogenized in SDS-PAGE sample buffer.
Related Product 1: PRMT5 (pT634) antibody blocking peptide (Cat. No.: PE-04ARI)
Scientific Background: PRMT5 (Protein Arginine Methyltransferase 5) is a highly conserved enzyme that acts as a key epigenetic regulator by catalyzing the symmetric dimethylation of arginine residues on histone and non-histone proteins. It plays a crucial role in gene expression, RNA processing, and cell growth, with high expression linked to various cancers. It targets histones (such as H3R8, H4R3) and transcription factors (like NFkB, p53). It can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA), with a preference for the formation of MMA. PRMT5 is essential for embryonic development, spliceosome assembly, ribosome biogenesis, and cell cycle regulation. It is found in both the cytoplasm and nucleus, with its location influencing its target substrates. It acts as an oncogene by inhibiting tumor suppressor genes (e.g., ST7, NM23) and aiding cancer cell survival and proliferation. This description may include information annotated by UniProt and/or Google AI.