Product Name:
PCYT1A-pT342+pS343
Product Number:
ab-pn547
Target Full Name: CTP: phosphocholine cytidylyltransferase isoform A
Target Alias: CCT A; CCT-alpha; CCTA; Choline-phosphate cytidylyltransferase A; CT; CT A; CTA; CTP:phosphocholine cytidylyltransferase A; CTPCT; PCY1A; PCYT1; PCYT1A; phosphate cytidylyltransferase 1, choline, alpha; Phosphorylcholine transferase A
Product Type Specific: PCYT1A phosphosite-specific antibody
Antibody Code: PN547
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T342+S343
Protein UniProt: P49585
Protein SigNET: PCYT1A
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human PCYT1A; CTPCT; CCTA sequence peptide Cat. No.: PE-04ARG95
Antibody Immunogen Sequence: FSGK(pT)(pS)PP(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues F338 to C346; Near the C-terminus of the protein. The effects of T342 and S343 phosphorylation are unclear. These are among the main in vivo phosphorylation sites in PCYT1A (≥33 and ≥50 reports, respectively, from high throughput mass spectrometry studies recorded in PhosphoSitePlus).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Dog | Rat | Mouse.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 40-45 kDa.
Antibody Specificity: Very high
Antibody Cross Reactivity: No significant cross-reactive proteins detected in phenylarsine oxide (PAO)+vanadate-treated HeLa cells, EGF-treated A431 cells and insulin-treated MCF7 cells, when these cells were homogenized in SDS-PAGE sample buffer.
Related Product 1: PCYT1A (338-346) pT342+pS343 antibody blocking peptide (Cat. No.: PE-04ARG95)
Related Product 2: PCYT1A expression antibody (Cat. No.: AB-NN606-1)
Related Product 3: PCYT1A Y359+S362 phosphorylation antibody (Cat. No.: AB-PN548)
Related Product 4: PCYT1A S329+S331 phosphorylation antibody (Cat. No.: AB-PN561)
Related Product 5: PCYT1B expression antibody (Cat. No.: AB-NN605-1)
Related Product 6: PCYT1B S315+S319 phosphorylation antibody (Cat. No.: AB-PN546)
Scientific Background: PCYT1A (Choline-phosphate cytidylyltransferase A; CCT-a) is the rate limiting 42 kDa enzyme in the biosynthetic pathway for phosphatidylcholine. It catalyzes the reaction: CTP + phosphocholine = diphosphate + CDP-choline. It is primarily localized to the nucleoplasm but moves to the inner nuclear membrane when activated by membrane tension, lipid imbalance and choline deficiency. It acts as a sensor for membrane lipid stored curvature elastic stress, triggering PC synthesis to repair membrane damage. Diacylglycerol, acidic phospholipids and fatty acids drive the binding of PCYT1A to the inner nuclear membrane and stimulation of its catalytic activity. It consists of a catalytic domain, a membrane-binding domain (amphipathic helix), and an autoinhibitory domain. It is crucial for proper lung development and surfactant production, with knockouts in mice causing severe respiratory distress and death shortly after birth. The protein is expressed ubiquitously, with high activity in tissues with high lipid turnover. Mutations in the PCYT1A gene are linked to rare autosomal-recessive disorders, specifically Spondylometaphyseal Dysplasia with Cone-Rod Dystrophy (SMDCRD) and, in some cases, Leber Plus Disease. This description may include information annotated by UniProt and/or Google AI.