Product Name:
PCYT1A-pT342+pS343
Product Number:
ab-pn547
Target Full Name: CTP: phosphocholine cytidylyltransferase isoform A
Target Alias: CCT A; CCT-alpha; CCTA; Choline-phosphate cytidylyltransferase A; CT; CT A; CTA; CTP:phosphocholine cytidylyltransferase A; CTPCT; PCY1A; PCYT1; PCYT1A; phosphate cytidylyltransferase 1, choline, alpha; Phosphorylcholine transferase A
Product Type Specific: PCYT1A phosphosite-specific antibody
Antibody Code: PN547
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T342+S343
Protein UniProt: P49585
Protein SigNET: P49585
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human PCYT1A; CTPCT; CCTA sequence peptide Cat. No.: PE-04ARG95
Antibody Immunogen Sequence: FSGK(pT)(pS)PP(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues F338 to C346; Near the C-terminus of the protein. These are among the main in vivo phosphorylation sites in PCYT1A.
Production Method: Corresponds to amino acid residues F338 to C346; Near the C-terminus of the protein. These are among the main in vivo phosphorylation sites in PCYT1A.
Antibody Modification: PCYT1A phosphosite-specific antibody
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 40-45 kDa.
Antibody Specificity: Very high
Antibody Cross Reactivity: No significant cross-reactive proteins detected in phenylarsine oxide (PAO)+vanadate-treated HeLa cells, EGF-treated A431 cells and insulin-treated MCF7 cells, when these cells were homogenized in SDS-PAGE sample buffer.
Related Product 1: PCYT1A-pT342+pS343 blocking peptide
Related Product 2: PCYT1A-pS329+pS331 phosphosite-specific antibody (Cat. No.: AB-PN561)
Related Product 3: PCYT1A-pY359+pS362 phosphosite-specific antibody (Cat. No.: AB-PN548)
Related Product 4: PCYT1B-pS315+pS319 phosphosite-specific antibody (Cat. No.: AB-PN546)
Scientific Background: PCYT1A is the rate limiting enzyme in the biosynthetic pathway for phosphatidylcholine. It catalyzes the reaction: CTP + phosphocholine = diphosphate + CDP-choline. It is an ambiquitous enzyme that resides in the cytoplasm of cells in an inactive form. Diacylglycerol, acidic phospholipids and fatty acids drive the binding of PCYT1A to the endoplasmic reticulum and stimulation of its catalytic activity.
