Product Name:
PCYT1B-pS315+pS319
Product Number:
ab-pn546
Target Full Name: CTP: phosphocholine cytidylyltransferase isoform B
Target Alias: CCT B; CCT-beta; CCTB; Choline-phosphate cytidylyltransferase B; CT B; CTB; CTP:phosphocholine cytidylyltransferase B; PCY1B; PCYT1B; phosphate cytidylyltransferase 1, choline, beta; Phosphorylcholine transferase B
Product Type Specific: PCYT1B phosphosite-specific antibody
Antibody Code: PN546
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S315+S319
Protein UniProt: Q9Y5K3
Protein SigNET: PCYT1B
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human PCYT1B; CCTB sequence peptide Cat. No.: PE-04ARF95
Antibody Immunogen Sequence: LQAL(pS)PKQ(pS)PV(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues L311 to V321; Near the C-terminus of the protein. S315 phosphorylation inhibits cytidyltransferase activity. S315 and S319 are the main in vivo phosphorylation sites in PCYT1B (≥36 and ≥9 reports, respectively, from high throughput mass spectrometry studies recorded in PhosphoSitePlus). PCYT1B is known to be phosphorylated in vitro at S315 by ERK1 (MAPK3), and ERK2 (MAPK1).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Medium immunoreactivity of a target-sized protein by Western blotting in A431, HeLa and MCF7 cells.
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Dog | Rat | Mouse | Platypus | Chicken | Frog | Zebra fish.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 40-52 kDa.
Antibody Specificity: Very high
Antibody Cross Reactivity: No significant cross-reactive proteins detected in A431, HeLa and MCF7 cells.
Related Product 1: PCYT1B (311-321) pS315+pS319 antibody blocking peptide (Cat. No.: PE-04ARF95)
Related Product 2: PCYT1A expression antibody (Cat. No.: AB-NN606-1)
Related Product 3: PCYT1A T342+S343 phosphorylation antibody (Cat. No.: AB-PN547)
Related Product 4: PCYT1A Y359+S362 phosphorylation antibody (Cat. No.: AB-PN548)
Related Product 5: PCYT1A S329+S331 phosphorylation antibody (Cat. No.: AB-PN561)
Related Product 6: PCYT1B expression antibody (Cat. No.: AB-NN605-1)
Scientific Background: PCYT1B (Phosphate cytidylyltransferase 1B, choline) is the rate limiting enzyme in the biosynthetic pathway for phosphatidylcholine. It catalyzes the reaction: CTP + phosphocholine = diphosphate + CDP-choline. It is an ambiquitous enzyme that resides in the cytoplasm of cells in an inactive form. Diacylglycerol, acidic phospholipids and fatty acids drive the binding of PCYT1B to the endoplasmic reticulum and stimulation of its catalytic activity. It is critical for membrane synthesis and lipid metabolism, with high expression in the brain, placenta, ocary, testis, and fetus. It belongs to the cytidylyltransferase family and possesses a catalytic core and helical domain nearly identical to PCYT1A, but lacks the specific nuclear localization signal found in PCYT1A. Multiple alternative splicing variants exist. The protein can be expressed in 35-kD and 42-kD forms, with 42-kD potentially being a post-translationally modified version. It is often found in the cytoplasm and associates with the endoplasmic reticulum. It plays a role in phosphatidylcholine compositional homeostasis, and has been associated with studies on neonatal respiratory distress syndrome and preeclampsia. This description may include information annotated by UniProt and/or Google AI.