Product Name:
PCYT1B-pS315+pS319
Product Number:
ab-pn546
Target Full Name: CTP: phosphocholine cytidylyltransferase isoform B
Target Alias: CCT B; CCT-beta; CCTB; Choline-phosphate cytidylyltransferase B; CT B; CTB; CTP:phosphocholine cytidylyltransferase B; PCY1B; PCYT1B; phosphate cytidylyltransferase 1, choline, beta; Phosphorylcholine transferase B
Product Type Specific: PCYT1B phosphosite-specific antibody
Antibody Code: PN546
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S315+S319
Protein UniProt: Q9Y5K3
Protein SigNET: Q9Y5K3
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human PCYT1B; CCTB sequence peptide Cat. No.: PE-04ARF95
Antibody Immunogen Sequence: LQAL(pS)PKQ(pS)PV(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues L311 to V321; Near the C-terminus of the protein. This is the main in vivo phosphorylation site in PCYT1B.
Production Method: Corresponds to amino acid residues L311 to V321; Near the C-terminus of the protein. This is the main in vivo phosphorylation site in PCYT1B.
Antibody Modification: PCYT1B phosphosite-specific antibody
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Medium immunoreactivity of a target-sized protein by Western blotting in A431, HeLa and MCF7 cells.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 40-52 kDa.
Antibody Specificity: Very high
Antibody Cross Reactivity: No significant cross-reactive proteins detected in A431, HeLa and MCF7 cells.
Related Product 1: PCYT1B-pS315+pS319 blocking peptide
Related Product 2: PCYT1A-pS329+pS331 phosphosite-specific antibody (Cat. No.: AB-PN561)
Related Product 3: PCYT1A-pT342+pS343 phosphosite-specific antibody (Cat. No.: AB-PN547)
Related Product 4: PCYT1A-pY359+pS362 phosphosite-specific antibody (Cat. No.: AB-PN548)
Scientific Background: PCYT1B is the rate limiting enzyme in the biosynthetic pathway for phosphatidylcholine. It catalyzes the reaction: CTP + phosphocholine = diphosphate + CDP-choline. It is an ambiquitous enzyme that resides in the cytoplasm of cells in an inactive form. Diacylglycerol, acidic phospholipids and fatty acids drive the binding of PCYT1B to the endoplasmic reticulum and stimulation of its catalytic activity.
