Product Name:
WASP-pY291
Product Number:
ab-pn545
Target Full Name: Wiskott-Aldrich syndrome protein
Product Type Specific: WASP phosphosite-specific antibody
Antibody Code: PN545
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y291
Protein UniProt: P42768
Protein SigNET: WASP
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human WASP sequence peptide Cat. No.: PE-04AFC99
Antibody Immunogen Sequence: KLI(pY)DFI(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues K288 to I294; in the PBD domain. Y291 phosphorylation induces activation of WASP to regulate cell cytoskeleton and induce cell motility. This is the major site of in vivo phosphorylation of WASP based on ≥291 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus. WASP is known to be phosphorylated at this site in vitro by Abl1, Ack, Btk, Fyn, Hck, Lck, Lyn, and NPM-ALK.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus Macaque | Dog | Rat | Mouse | Chicken | Frog | Zebra fish | Fruit fly.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 54-62 kDa.
Antibody Specificity: Very high
Antibody Cross Reactivity: No significant cross-reactive proteins in sea star oocytes.
Related Product 1: WASP (288-294) pY291 antibody blocking peptide (Cat. No.: PE-04AFC99)
Scientific Background: WASP is a member of the Wiskott-Aldrich syndrome (WAS) family of proteins. It is a cytoplasmic protein expressed exclusively in hematopoietic cells. WASP transduces signals from surface receptors to the actin cytoskeleton. It associates with the small GTPase, Cdc42, to regulate formation of actin filaments, and the cytoskeletal organizing complex, Arp2/3. It is mutated in Wiskott-Aldrich syndrome,which is a rare, inherited, X-linked, recessive disease characterized by immune dysregulation and microthrombocytopenia. This description may include information annotated by UniProt and/or Google AI.