Product Name:
VIM-pY117
Product Number:
ab-pn544
Target Full Name: Vimentin
Target Alias: FLJ36605; VIME
Product Type Specific: VIM phosphosite-specific antibody
Antibody Code: PN544
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y117
Protein UniProt: P08670
Protein SigNET: VIM
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human VIM sequence peptide Cat. No.: PE-04AEW99
Antibody Immunogen Sequence: FAN(pY)IDK(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues F114 to K120; in the N-terminal part of the Filament domain. The effect of Y117 phosphorylation is unclear. This is one of the major in vivo phosphorylation sites in vimentin based on ≥672 mass spectrometry reports recorded in PhosphoSitePlus.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.75 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Medium-strong immunoreactivity of a target-sized protein by Western blotting inn A431 cells. Strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus Macaque | Dog | Rat | Mouse | Chicken.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 50-57 kDa.
Antibody Specificity: Medium-High
Antibody Cross Reactivity: No significant cross-reactive proteins detected in A431, HeLa MCF7 and T98G cells and sea star oocytes, except for 65 and 140 kDa cross-reactive proteins in A431, MCF7 and T98G cells, and a strongly reactive 35 kDa protein in T98G cells.
Related Product 1: VIM (114-120) pY117 antibody blocking peptide (Cat. No.: PE-04AEW99)
Scientific Background: Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is found in the nucleus, endoplasmic reticulum, and mitochondria, attached either laterally or terminally. It is involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2. This description may include information annotated by UniProt and/or Google AI.