Product Name:
VIM-pY117
Product Number:
ab-pn544
Target Full Name: Vimentin
Target Alias: FLJ36605; VIME
Product Type Specific: VIM phosphosite-specific antibody
Antibody Code: PN544
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y117
Protein UniProt: P08670
Protein SigNET: P08670
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human VIM sequence peptide Cat. No.: PE-04AEW99
Antibody Immunogen Sequence: FAN(pY)IDK(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues F114 to K120; In the N-terminal part of the Filament domain. One of the major in vivo phosphorylation sites in VIM.
Production Method: Corresponds to amino acid residues F114 to K120; In the N-terminal part of the Filament domain. One of the major in vivo phosphorylation sites in VIM.
Antibody Modification: VIM phosphosite-specific antibody
Antibody Concentration: 0.75 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Medium-strong immunoreactivity of a target-sized protein by Western blotting inn A431 cells. Strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 50-57 kDa.
Antibody Specificity: Medium-High
Antibody Cross Reactivity: No significant cross-reactive proteins detected in A431, HeLa MCF7 and T98G cells and sea star oocytes, except for 65 and 140 kDa cross-reactive proteins in A431, MCF7 and T98G cells, and a strongly reactive 35 kDa protein in T98G cells.
Related Product 1: VIM-pY117 blocking peptide
Scientific Background: Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is found in the nucleus, endoplasmic reticulum, and mitochondria, attached either laterally or terminally. It is involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.
