Product Name:
Vav1-pY826
Product Number:
ab-pn543
Target Full Name: Vav 1 guanine nucleotide exchange factor
Target Alias: Vav; vav 1 guanine nucleotide exchange factor; Vav proto-oncogene
Product Type Specific: Vav1 phosphosite-specific antibody
Antibody Code: PN543
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y826
Protein UniProt: P15498
Protein SigNET: P15498
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human Vav1 sequence peptide Cat. No.: PE-04AEO99
Antibody Immunogen Sequence: GEI(pY)GRV(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues G823 to V829; In the SH3_1 domain
Production Method: Corresponds to amino acid residues G823 to V829; In the SH3_1 domain
Antibody Modification: Vav1 phosphosite-specific antibody
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Medium immunoreactivity of a target-sized protein by Western blotting in Jurkat cells. Medium immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 100-110 kDa.
Antibody Specificity: High
Antibody Cross Reactivity: No significant cross-reactive proteins detected in HeLa cells and sea star oocytes. ~45-47 kDa and ~80 kDa cross-reactive proteins were also detectable in Jurkat cells.
Related Product 1: Vav1-pY826 blocking peptide
Scientific Background: Vav1 is a proto-oncogenic guanine nucleotide exchange factors (GEF) of the Dbl family that couples receptor-protein-tyrosine kinase signals with the activation of the Rho/Rac GTPases to lead to cell differentiation and/or proliferation. It is expressed exclusively in hematopoietic cells and is important in hematopoiesis, by playing roles in T-cell and B-cell development and activation.
