Product Name:
Vav1-pY826
Product Number:
ab-pn543
Target Full Name: Vav 1 guanine nucleotide exchange factor
Target Alias: Vav; vav 1 guanine nucleotide exchange factor; Vav proto-oncogene
Product Type Specific: VAV1 phosphosite-specific antibody
Antibody Code: PN543
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y826
Protein UniProt: P15498
Protein SigNET: Vav1
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human Vav1 sequence peptide Cat. No.: PE-04AEO99
Antibody Immunogen Sequence: GEI(pY)GRV(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues G823 to V829; in the SH3_1 domain. Y826 phosphorylation regulates carcinogenesis, cell growth, activation and molecular associations, including inducing interaction with Abl, CSK, SH2D1A, and Shc1. This is one of the major sites of in vivo phosphorylation of Vav based on ≥95 mass spectrometry reports recorded in PhosphoSitePlus.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Medium immunoreactivity of a target-sized protein by Western blotting in Jurkat cells. Medium immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Dog | Rat | Mouse | Frog.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 100-110 kDa.
Antibody Specificity: High
Antibody Cross Reactivity: No significant cross-reactive proteins detected in HeLa cells and sea star oocytes. ~45-47 kDa and ~80 kDa cross-reactive proteins were also detectable in Jurkat cells.
Related Product 1: VAV1 (823-829) pY826 antibody blocking peptide (Cat. No.: PE-04AEO99)
Scientific Background: Vav1 is a proto-oncogenic guanine nucleotide exchange factors (GEF) of the Dbl family that couples receptor-protein-tyrosine kinase signals with the activation of the Rho/Rac GTPases to lead to cell differentiation and/or proliferation. It drives actin cytoskeleton rearrangement, T-cell/B-cell development, and activation. It functions as a molecular switch, regulated by tyrosine phosphorylation to initiate signaling cascades for immunological synapse assembly, calcium mobilization, and transcriptional regulation. It is expressed exclusively in hematopoietic cells and is important in hematopoiesis, by playing roles in T-cell and B-cell development and activation. It acts as a GEF for Rho family GTPases (e.g., Rac1, RhoA, Cdc42) by converting GDP to GTP, and as a scaffold protein to assemble multi-protein signaling complexes. Vav1 features a complex structure including DH (DBL homology), PH (pleckstrin homology), SH2 (Src homology 2), two SH3 domains, and an actin-binding CH domain. Its dysfunction is linked to autoimmune diseases and T-cell malignancies. It is a binding partner of Nef proteins from HIV-1, and facilitates viral replication. This description may include information annotated by UniProt and/or Google AI.