Product Name:
TLN1-pY70
Product Number:
ab-pn542
Target Full Name: Talin 1
Target Alias: OTTMUSP00000007396; Talin-1; Tln; Tln1
Product Type Specific: TLN1 phosphosite-specific antibody
Antibody Code: PN542
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y70
Protein UniProt: Q9Y490
Protein SigNET: Q9Y490
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human TLN1 sequence peptide Cat. No.: PE-04ADP85
Antibody Immunogen Sequence: ALD(pY)YML(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues A67 to L73; In the N-terminus of the protein. This is the major in vivo phosphorylation site in TLN1.
Production Method: Corresponds to amino acid residues A67 to L73; In the N-terminus of the protein. This is the major in vivo phosphorylation site in TLN1.
Antibody Modification: TLN1 phosphosite-specific antibody
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 260-280 kDa.
Related Product 1: TLN1-pY70 blocking peptide
Scientific Background: Talin-1 is a cytoskeletal protein that is found in areas of cell-substratum and cell-cell contacts. It plays a significant role in the assembly of actin filaments and in spreading and migration of various cell types, including fibroblasts and osteoclasts. This protein interacts with integrins in the cell surface membrane in order to assist in the attachment of adherent cells to extracellular matrices and of lymphocytes to other cells. The N-terminus of this protein facilitates localization to cell-extracellular matrix junction, whereas the C-terminus contains binding sites for proteins such as beta-1-integrin, actin, and vinculin.
