Product Name:
STAT3-pY705+pT708
Product Number:
ab-pn539
Target Full Name: Signal transducer and activator of transcription 3
Target Alias: Acute-phase response factor; APRF
Product Type Specific: STAT3 phosphosite-specific antibody
Antibody Code: PN539
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y705+T708
Protein UniProt: P40763
Protein SigNET: STAT3
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human STAT3 sequence peptide Cat. No.: PE-04ADO95
Antibody Immunogen Sequence: AAP(pY)LK(pT)(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues A702 to T708; in the C-terminus of the transcription factor. Y705 and Y708 phosphorylation stimulates transcriptional activity, induces interaction with DNA, PKCD, and STAT1, and regulates apoptosis, cell adhesion, cell differentiation, cell growth, cell motility, transcription, activation, intracellular location, and protein degradation. These are major sites of in vivo phosphorylation of STAT3 (≥3122 and ≥161 reports, respectively, from high throughput mass spectrometry studies recorded in PhosphoSitePlus). STAT3 is known to be phosphorylated in vitro at Y705 by Alk, Axl, EphB1, Fer, JAK1, JAK2, MAPKAPK2, MEKK1 (MAP3K1), MER (MERTK), PKM2 (PKM), Ret iso3, and Src.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.75 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Dog | Rat | Mouse | Chicken | Frog | Zebra fish.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 80-88 kDa.
Antibody Specificity: High
Antibody Cross Reactivity: Almost no significant cross-reactivities detected in HepG2 and T98G cells.
Related Product 1: STAT3 (702-708) pY705+pT708 antibody blocking peptide (Cat. No.: PE-04ADO95)
Related Product 2: STAT1 Y701 phosphorylation antibody (Cat. No.: AB-PN666)
Related Product 3: STAT1 S727 phosphorylation antibody (Cat. No.: AB-PN667)
Related Product 4: STAT2 Y690 phosphorylation antibody (Cat. No.: AB-PN668)
Related Product 5: STAT3 S727 phosphorylation antibody (Cat. No.: AB-PN669)
Related Product 6: STAT4 Y693 phosphorylation antibody (Cat. No.: AB-PN670)
Related Product 7: STAT5A Y694 phosphorylation antibody (Cat. No.: AB-PN671)
Related Product 8: STAT5A S780 phosphorylation antibody (Cat. No.: AB-PN672)
Related Product 9: STAT6 Y641 phosphorylation antibody (Cat. No.: AB-PN673)
Scientific Background: STAT3 (Signal transducer and activator of transcription 3) is a transcription factor of the STAT family that mediates cellular responses to interleukins, KITLG/SCF, LEP and other growth factors. Once activated, it recruits coactivators, such as NCOA1 or MED1, to the promoter region of the target gene (PubMed:17344214). It mediates cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. It also binds to the interleukin-6 (IL-6)-responsive elements identified in the promoters of various acute-phase protein genes. This description may include information annotated by UniProt and/or Google AI.