Product Name:
STAT3-pY705+pT708
Product Number:
ab-pn539
Target Full Name: Signal transducer and activator of transcription 3
Target Alias: Acute-phase response factor; APRF
Product Type Specific: STAT3 phosphosite-specific antibody
Antibody Code: PN539
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y705+T708
Protein UniProt: P40763
Protein SigNET: P40763
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human STAT3 sequence peptide Cat. No.: PE-04ADO95
Antibody Immunogen Sequence: AAP(pY)LK(pT)(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues A702 to T708; In the C-terminus of the transcription factor. Y705 is the major in vivo phosphorylation site in STAT3.
Production Method: Corresponds to amino acid residues A702 to T708; In the C-terminus of the transcription factor. Y705 is the major in vivo phosphorylation site in STAT3.
Antibody Modification: STAT3 phosphosite-specific antibody
Antibody Concentration: 0.75 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 80-88 kDa.
Antibody Specificity: High
Antibody Cross Reactivity: Almost no significant cross-reactivities detected in HepG2 and T98G cells.
Related Product 1: STAT3-pY705+pT708 blocking peptide
Scientific Background: STAT3 is a Signal transducer and transcription activator that mediates cellular responses to interleukins, KITLG/SCF, LEP and other growth factors. Once activated, it recruits coactivators, such as NCOA1 or MED1, to the promoter region of the target gene (PubMed:17344214). It mediates cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. It also binds to the interleukin-6 (IL-6)-responsive elements identified in the promoters of various acute-phase protein genes.
