Product Name:
STAM2-pY374
Product Number:
ab-pn538
Target Full Name: Signal transducing adapter molecule 2
Target Alias: DKFZp564C047; HBP; Hrs-binding protein; HSE1 homolog; Signal transducing adapter molecule 2; signal transducing adaptor molecule (SH3 domain and ITAM motif) 2; STAM-2; STAM-like protein containing SH3 and ITAM domains 2; STAM2; STAM2A; STAM2B
Product Type Specific: STAM2 phosphosite-specific antibody
Antibody Code: PN538
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y374
Protein UniProt: O75886
Protein SigNET: STAM2
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human STAM2 sequence peptide Cat. No.: PE-04AEF95
Antibody Immunogen Sequence: YSV(pY)SKL(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues Y371 to L377; in the C-terminal half of the protein. This is the second major in vivo phosphorylation site in STAM2 (≥376 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus). The effect of Y374 phosphorylation is unclear. STAM2 is known to be phosphorylated at this site in vitro by Fyn.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.5 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rat | Mouse.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 65-72 kDa.
Related Product 1: STAM2 (371-377) pY374 antibody blocking peptide (Cat. No.: PE-04AEF95)
Scientific Background: STAM2 (Signal Transducing Adaptor Molecule 2; STAMP2) is a multifunctional, tyrosine-phosphorylated protein that serves as a key component of the ESCRT-0 complex (endosomal sorting complex required for transport). It is primarily localized to early endosomes, and STAM2 also acts in the cytoplasm and nucleus, particularly in neurons. It contains structural domains that facilitate binding to other proteins and ubiquitin, specifically the VHS, UIM, and SH3 domains .It plays a critical role in trafficking and sorting ubiquitinated receptors, specifically guiding activated receptors toward lysosomal degradation.Upon IL-2 and GM-CSL stimulation, it plays a role in signalling leading to DNA synthesis and MYC induction. It may also function in T-cell development, and appears to be involved in down-regulation of receptor-tyrosine kinases via multivesicular body (MVBs) when complexed with HGS (ESCRT-0 complex). It regulates intracellular signaling, including the suppression of Akt activation through interactions with PTP1B, and supports EGFR degradation by interacting with Rin1. STAM2 acts downstream of JAK kinases, and its phosphorylation is induced by cytokines and growth factors. This description may include information annotated by UniProt and/or Google AI.