Product Name:
snRNP 70-pY126
Product Number:
ab-pn537
Target Full Name: U1 small nuclear ribonucleoprotein 70 kDa
Target Alias: RNPU1Z; RPU1; RU17; small nuclear ribonucleoprotein 70kDa (U1); Snp1; SNRNP70; SNRP70; U1 small nuclear ribonucleoprotein 70 kDa; U1 snRNP 70 kDa; U1-70K; U170K; U1AP; U1AP1; U1RNP
Product Type Specific: snRNP70 phosphosite-specific antibody
Antibody Code: PN537
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y126
Protein UniProt: P08621
Protein SigNET: snRNP 70
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human snRNP 70 sequence peptide Cat. No.: PE-04AFF99
Antibody Immunogen Sequence: FEV(pY)GPI(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues F123 to I129; in the RRM_1 domain. The effect of Y126 phosphorylation is unclear. This is the major sites of in vivo phosphorylation of snRNP70 based on ≥643 mass spectrometry reports recorded in PhosphoSitePlus.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus Macaque | Dog | Rat | Mouse | Frog | Fruit fly | Honey bee | Sea urchin | Thale cress |.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 60-65 kDa.
Antibody Specificity: Very high
Antibody Cross Reactivity: No significant cross-reactive proteins detected in HeLa and T98G cells.
Related Product 1: snRNP70 (123-129) pY126 antibody blocking peptide (Cat. No.: PE-04AFF99)
Scientific Background: SNRNP70 is part of the spliceosomal U1 snRNP, which is essential for recognition of the pre-mRNA 5' splice-site and spliceosome assembly. It is a critical RNA-binding protein that acts as a core subunit of the U1 small nuclear ribonucleoprotein (U1 snRNP) complex, initiating pre-mRNA splicing by recognizing the 5' splice site. SNRNP70 binds to the loop I region of U1-snRNA. It features an RNA recognition motif (RRM), an α-helical domain, and arginine/serine-rich (RS) domains that facilitate interactions with other splicing factors (e.g., SRSF1). SNRNP70 is primarily located in the nucleoplasm, though it is found in the cytoplasm during certain phases. It is linked to autoimmune diseases such as mixed connective tissue disease (MCTD) and systemic lupus erythematosus (where it acts as an autoantigen). It forms detergent-insoluble aggregates in Alzheimer's disease and co-localizes with Tau, making it a biomarker for the condition. This description may include information annotated by UniProt and/or Google AI.