Product Name:
SIT-pY95
Product Number:
ab-pn536
Target Full Name: Signaling threshold-regulating transmembrane adapter 1
Target Alias: gp30/40; MGC125908; MGC125909; MGC125910; RP11-331F9.5; SHP2 interacting transmembrane adaptor; SHP2-interacting transmembrane adapter protein; SHP2-interacting transmembrane adaptor protein; signaling threshold regulating transmembrane adaptor 1; Signaling threshold-regulating transmembrane adapter 1; SIT; SIT1; suppression inducing transmembrane adaptor 1; Suppression-inducing transmembrane adapter 1
Product Type Specific: SIT phosphosite-specific antibody
Antibody Code: PN536
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y95
Protein UniProt: Q9Y3P8
Protein SigNET: SIT
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human SIT sequence peptide Cat. No.: PE-04AHF95
Antibody Immunogen Sequence: NLH(pY)LQT(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues N92 to T98; in the middle of the SIT domain I the protein. The effect of Y95 phosphorylation is unclear. This is one of the major in vivo phosphorylation sites in SIT based on ≥123 mass spectrometry reports reported in PhosphoSitePlus.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Rhesus Macaque | Dog | Rat | Mouse | Platypus.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 19-21 kDa.
Antibody Specificity: Very high
Antibody Cross Reactivity: Almost no significant cross-reactivities detected in HeLa cells, except phenylarsine oxide (PAO) increases 25 kDa protein; No significant cross-reactivities detected in MCF7 and T98G cells.
Related Product 1: SIT (92-98) pY95 antibody blocking peptide (Cat. No.: PE-04AHF95)
Related Product 2: SIT-pY90 phosphosite-specific antibody (Cat. No.: AB-PN535)
Scientific Background: SIT (Signaling Threshold-Regulating Transmembrane Adapter Protein) negatively regulates TCR (T-cell antigen receptor)-mediated signalling in T-cells. It is involved in positive selection of T-cells. SIT-deficient mice showed that the absence of SIT leads to T-cell hyperresponsiveness and higher production of T-helper 1 (TH1) cytokines. It contains tyrosine-based signaling motifs (TBSMs) in its cytoplasmic tail. When phosphorylated, these motifs allow SIT to recruit inhibitory molecules (such as the phosphatase SHP-2) to the TCR complex, dampening activation signals. SIT helps set the activation threshold during T-cell selection in the thymus, preventing premature activation. Unlike many other adaptors found in lipid rafts, SIT is largely excluded from the lipid raft fraction of the plasma membrane. It plays a role in preventing autoimmunity; SIT-deficient mice display increased susceptibility to developing experimental autoimmune encephalomyelitis. This description may include information annotated by UniProt and/or Google AI.