Product Name:
SIT-pY95
Product Number:
ab-pn536
Target Full Name: Signaling threshold-regulating transmembrane adapter 1
Target Alias: gp30/40; MGC125908; MGC125909; MGC125910; RP11-331F9.5; SHP2 interacting transmembrane adaptor; SHP2-interacting transmembrane adapter protein; SHP2-interacting transmembrane adaptor protein; signaling threshold regulating transmembrane adaptor 1; Signaling threshold-regulating transmembrane adapter 1; SIT; SIT1; suppression inducing transmembrane adaptor 1; Suppression-inducing transmembrane adapter 1
Product Type Specific: SIT phosphosite-specific antibody
Antibody Code: PN536
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y95
Protein UniProt: Q9Y3P8
Protein SigNET: Q9Y3P8
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human SIT sequence peptide Cat. No.: PE-04AHF95
Antibody Immunogen Sequence: NLH(pY)LQT(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues N92 to T98; In the middle of the SIT domain I the protein. One of the major in vivo phosphorylation sites in SIT.
Production Method: Corresponds to amino acid residues N92 to T98; In the middle of the SIT domain I the protein. One of the major in vivo phosphorylation sites in SIT.
Antibody Modification: SIT phosphosite-specific antibody
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 19-21 kDa.
Antibody Specificity: Very high
Antibody Cross Reactivity: Almost no significant cross-reactivities detected in HeLa cells, except phenylarsine oxide (PAO) increases 25 kDa protein; No significant cross-reactivities detected in MCF7 and T98G cells.
Related Product 1: SIT-pY95 blocking peptide
Related Product 2: SIT-pY90 phosphosite-specific antibody (Cat. No.: AB-PN535)
Scientific Background: SIT negatively regulates TCR (T-cell antigen receptor)-mediated signalling in T-cells. It is involved in positive selection of T-cells.
