Product Name:
SIT-pY90
Product Number:
ab-pn535
Target Full Name: Signaling threshold-regulating transmembrane adapter 1
Target Alias: gp30/40; MGC125908; MGC125909; MGC125910; RP11-331F9.5; SHP2 interacting transmembrane adaptor; SHP2-interacting transmembrane adapter protein; SHP2-interacting transmembrane adaptor protein; signaling threshold regulating transmembrane adaptor 1; Signaling threshold-regulating transmembrane adapter 1; SIT; SIT1; suppression inducing transmembrane adaptor 1; Suppression-inducing transmembrane adapter 1
Product Type Specific: SIT phosphosite-specific antibody
Antibody Code: PN535
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y90
Protein UniProt: Q9Y3P8
Protein SigNET: Q9Y3P8
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human SIT sequence peptide Cat. No.: PE-04AEL99
Antibody Immunogen Sequence: VPL(pY)GNL(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues V87 to L93; In the Sit domain. This is the major in vivo phosphorylation site of SIT.
Production Method: Corresponds to amino acid residues V87 to L93; In the Sit domain. This is the major in vivo phosphorylation site of SIT.
Antibody Modification: SIT phosphosite-specific antibody
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 19-21 kDa.
Antibody Specificity: Very high
Antibody Cross Reactivity: No significant cross-reactive proteins detected in HepG2, Jurkat, MCF7, and T98G cells as well as in sea star oocytes.
Related Product 1: SIT-pY90 blocking peptide
Related Product 2: SIT-pY95 phosphosite-specific antibody (Cat. No.: AB-PN536)
Scientific Background: SIT negatively regulates TCR (T-cell antigen receptor)-mediated signalling in T-cells. It is involved in positive selection of T-cells.
