Product Name:
SHIP2-pY886
Product Number:
ab-pn534
Target Full Name: Phosphatidylinositol-3,4,5-trisphosphate 5-phosphatase 2
Target Alias: 51C protein; Inositol polyphosphate 5-phosphatase; Inositol polyphosphate phosphatase-like 1; SH2-containing inositol 5-phosphatase 2; SHIP2
Product Type Specific: SHIP2 (INPPL1) phosphosite-specific antibody
Antibody Code: PN534
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y886
Protein UniProt: O15357
Protein SigNET: SHIP2
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human SHIP2 (INPPL1) sequence peptide Cat. No.: PE-04AES99
Antibody Immunogen Sequence: ERL(pY)EWI(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues E883 to I889; in the region between the Exo-endo_phos and SHAM_1 domains. The effect of Y886 phosphorylation is unclear. This is one of the major sites of in vivo phosphorylation of SHIP2 based on ≥578 mass spectrometry reports recorded in PhosphoSitePlus.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Medium immunoreactivity of a target-sized protein by Western blotting in HeLa cells.
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus Macaque | Dog | Rat | Mouse | Zebra fish.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 130-160 kDa.
Antibody Specificity: High-very high
Antibody Cross Reactivity: Almost no significant cross-reactivities detected in HeLa cells, except phenylarsine oxide (PAO) increases 140 + 55 kDa proteins; No significant cross-reactivities detected in HepG2 cells.
Related Product 1: SHIP2-pY886 blocking peptide
Related Product 2: SHIP2-1 pan-specific antibody (Cat. No.: AB-NP045-1)
Related Product 3: SHIP2-2 pan-specific antibody (Cat. No.: AB-NP045-2)
Related Product 4: SHIP2-3 pan-specific antibody (Cat. No.: AB-NP045-3)
Scientific Background: SHIP2 (Src homology 2 domain-containing inositol 5-phosphatase 2; INPPL1) is a 140 kDa phosphatidylinositol (PtdIns) phosphatase that specifically hydrolyzes the 5-phosphate of phosphatidylinositol-3,4,5-trisphosphate (PtdIns(3,4,5)P3) to produce PtdIns(3,4)P2, and negatively regulate the PI3K (phosphoinositide 3-kinase) pathways. It features an N-terminal SH2 domain, a central 5-phosphatase catalytic domain, and a C-terminal proline-rich domain (containing PXXP and NPXY motifs). SHIP2 interacts with proteins like filamin and vinexin to regulate cell spreading, adhesion, and endocytosis. It is essential for maintaining actin structure and membrane ruffle formation. SHIP2 is expressed ubiquitously, with high levels in the skeletal muscle, heart, and brain. While overexpression reduces both insulin-stimulated MAP kinase and Akt activation, its absence does not affect insulin signalling or GLUT4 trafficking. It confers resistance to dietary obesity. It may also hydrolyze PtdIns(1,3,4,5)P4. Mutations in SHIP2 are associated with opsismodysplasia, a skeletal development disorder. This description may include information annotated by UniProt and/or Google AI.