Product Name:
SHIP2-pY886
Product Number:
ab-pn534
Target Full Name: Phosphatidylinositol-3,4,5-trisphosphate 5-phosphatase 2
Target Alias: 51C protein; Inositol polyphosphate 5-phosphatase; Inositol polyphosphate phosphatase-like 1; SH2-containing inositol 5-phosphatase 2; SHIP2
Product Type Specific: SHIP2 (INPPL1) phosphosite-specific antibody
Antibody Code: PN534
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y886
Protein UniProt: O15357
Protein SigNET: O15357
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human SHIP2 (INPPL1) sequence peptide Cat. No.: PE-04AES99
Antibody Immunogen Sequence: ERL(pY)EWI(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues E883 to I889; In the region between the Exo-endo_phos and SHAM_1 domains. This is one of the main in vivo phosphorylation sites in proteins.
Production Method: Corresponds to amino acid residues E883 to I889; In the region between the Exo-endo_phos and SHAM_1 domains. This is one of the main in vivo phosphorylation sites in proteins.
Antibody Modification: SHIP2 (INPPL1) phosphosite-specific antibody
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Medium immunoreactivity of a target-sized protein by Western blotting in HeLa cells.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 130-160 kDa.
Antibody Specificity: High-very high
Antibody Cross Reactivity: Almost no significant cross-reactivities detected in HeLa cells, except phenylarsine oxide (PAO) increases 140 + 55 kDa proteins; No significant cross-reactivities detected in HepG2 cells.
Related Product 1: SHIP2-pY886 blocking peptide
Related Product 2: SHIP2-1 pan-specific antibody (Cat. No.: AB-NP045-1)
Related Product 3: SHIP2-2 pan-specific antibody (Cat. No.: AB-NP045-2)
Related Product 4: SHIP2-3 pan-specific antibody (Cat. No.: AB-NP045-3)
Scientific Background: SHIP2 (INPPL1) is a phosphatidylinositol (PtdIns) phosphatase that specifically hydrolyzes the 5-phosphate of phosphatidylinositol-3,4,5-trisphosphate (PtdIns(3,4,5)P3) to produce PtdIns(3,4)P2, and negatively regulate the PI3K (phosphoinositide 3-kinase) pathways. While overexpression reduces both insulin-stimulated MAP kinase and Akt activation, its absence does not affect insulin signalling or GLUT4 trafficking. It confers resistance to dietary obesity. It may also hydrolyze PtdIns(1,3,4,5)P4.
