Product Name:
PTRF-pY308
Product Number:
ab-pn533
Target Full Name: Polymerase I and transcript release factor
Target Alias: Cavin-1; Leucine-zipper protein FKSG13
Product Type Specific: PTRF phosphosite-specific antibody
Antibody Code: PN533
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y308
Protein UniProt: Q6NZI2
Protein SigNET: Q6NZI2
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human PTRF sequence peptide Cat. No.: PE-04AEP95
Antibody Immunogen Sequence: HVV(pY)ARS(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues H305 to S311
Production Method: Corresponds to amino acid residues H305 to S311
Antibody Modification: PTRF phosphosite-specific antibody
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 45-56 kDa.
Antibody Specificity: Very high
Antibody Cross Reactivity: No significant cross-reactive proteins detected in HeLa and T98G cells.
Related Product 1: PTRF-pY308 blocking peptide
Scientific Background: PTRF plays an important role in caveolae formation and organization. It is required for the sequestration of mobile caveolin into immobile caveolae. Termination of transcription by RNA polymerase I involves pausing of transcription by TTF1, and the dissociation of the transcription complex, releasing pre-rRNA and RNA polymerase I from the template. PTRF is required for dissociation of the ternary transcription complex.
