Product Name:
PTRF-pY308
Product Number:
ab-pn533
Target Full Name: Polymerase I and transcript release factor
Target Alias: Cavin-1; Leucine-zipper protein FKSG13
Product Type Specific: PTRF phosphosite-specific antibody
Antibody Code: PN533
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y308
Protein UniProt: Q6NZI2
Protein SigNET: PTRF
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human PTRF sequence peptide Cat. No.: PE-04AEP95
Antibody Immunogen Sequence: HVV(pY)ARS(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues H305 to S311. The effect of Y308 phosphorylation is unclear. This is one of the major sites of in vivo phosphorylation of PTPRF based on ≥1328 mass spectrometry reports recorded in PhosphoSitePlus.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus Macaque | Dog | Rat | Mouse | Chicken.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 45-56 kDa.
Antibody Specificity: Very high
Antibody Cross Reactivity: No significant cross-reactive proteins detected in HeLa and T98G cells.
Related Product 1: PTRF (305-311) pY308 antibody blocking peptide (Cat. No.: PE-04AEP95)
Related Product 2: PTPRA Y798 phosphorylation antibody (Cat. No.: AB-PP521)
Related Product 3: PTPRB Y1981 phosphorylation antibody (Cat. No.: AB-PP522)
Related Product 4: PTPRE Y696 phosphorylation antibody (Cat. No.: AB-PP554)
Related Product 5: PTPRF Y1621 phosphorylation antibody (Cat. No.: AB-PP523)
Related Product 6: PTPRK Y858 phosphorylation antibody (Cat. No.: AB-PP539)
Related Product 7: PTPRK Y916 phosphorylation antibody (Cat. No.: AB-PP524)
Related Product 8: PTPRM Y929 phosphorylation antibody (Cat. No.: AB-PP526)
Related Product 9: PTRF Y308 phosphorylation antibody (Cat. No.: AB-PN646)
Scientific Background: PTRF (Polymerase I and Transcript Release Factor; Cavin-1) is a critical protein for forming caveolae (small plasma membrane invaginations) and regulating ribosomal RNA (rRNA) transcription. It is required for the sequestration of mobile caveolin into immobile caveolae. It was originally identified as a factor that releases RNA polymerase I from the DNA template, terminating transcription. Termination of transcription by RNA polymerase I involves pausing of transcription by TTF1, and the dissociation of the transcription complex, releasing pre-rRNA and RNA polymerase I from the template. PTRF is required for dissociation of the ternary transcription complex. It is a soluble protein (approx. 44–52 kDa) with leucine zipper domains, a nuclear localization signal, and PEST sequences that regulate its degradation. PTRF is highly expressed in muscles, adipocytes, and lung tissue. Deficiency in PTRF leads to severe metabolic issues, including lipodystrophy, insulin resistance, and muscle dysfunction. This description may include information annotated by UniProt and/or Google AI.