Product Name:
PPP1R11-pY64
Product Number:
ab-pn532
Target Full Name: Protein phosphatase 1 regulatory subunit 11
Target Alias: HCG V; HCG-V; HCGV; Hemochromatosis candidate gene V protein; inhibitor-3; IPP3; MGC125741; MGC125742; MGC125743; PP1RB; PPP1R11; Protein phosphatase 1 regulatory subunit 11; protein phosphatase 1, regulatory (inhibitor) subunit 11; protein phosphatase 1, regulatory (inhibitor) subunit 11, isoform 1; Protein phosphatase inhibitor 3; t-complex-associated-testis-expressed 5; TCTE5; TCTEX5
Product Type Specific: PPP1R11 phosphosite-specific antibody
Antibody Code: PN532
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y64
Protein UniProt: O60927
Protein SigNET: PPP1R11
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human PPP1R11 (HCG V) sequence peptide Cat. No.: PE-04ADI99
Antibody Immunogen Sequence: CCI(pY)EKP(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues C61 to P67; in the PPI_Ypi1 domain. The effect of Y64 phosphorylation is unclear. This is a major in vivo phosphorylation site in PPP1R11 based on ≥474 mass spectrometry reports recorded in PhosphoSitePlus.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Dog | Rat | Mouse | Platypus | Frog | Zebra fish | Fruit fly | Red bread mold.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 18-23 kDa.
Antibody Specificity: High
Antibody Cross Reactivity: Almost no significant cross-reactivities detected in T98G cells.
Related Product 1: PPP1R11 (61-67) pY64 antibody blocking peptide (Cat. No.: PE-04ADI99)
Related Product 2: PPP1R12A Y496 phosphorylation antibody (Cat. No.: AB-PP543)
Related Product 3: PPP1R12A Y766 phosphorylation antibody (Cat. No.: AB-PP544)
Related Product 4: PPP1R12B T316 phosphorylation antibody (Cat. No.: AB-PP503)
Related Product 5: PPP1R14B T57 phosphorylation antibody (Cat. No.: AB-PP545)
Related Product 6: PPP1R14B Y29 phosphorylation antibody (Cat. No.: AB-PP546)
Related Product 7: PPP1R16A Y434 phosphorylation antibody (Cat. No.: AB-PP547)
Related Product 8: PPP1R1A T35 phosphorylation antibody (Cat. No.: AB-PP548)
Related Product 9: PPP1R1B S45+S46 phosphorylation antibody (Cat. No.: AB-PP558)
Scientific Background: PPP1R11 is a regulatory subunit for protein-serine/threonine phosphatase 1. It inhibits its phosphatase activity. Y64 is amonst the most commonly reported tyrosine phosphorylation sites in human proteins as observed by mass spectrometry. The role of this phosphorylation site is unclear. This description may include information annotated by UniProt and/or Google AI.