Product Name:
PPP1R11-pY64
Product Number:
ab-pn532
Target Full Name: Protein phosphatase 1 regulatory subunit 11
Target Alias: HCG V; HCG-V; HCGV; Hemochromatosis candidate gene V protein; inhibitor-3; IPP3; MGC125741; MGC125742; MGC125743; PP1RB; PPP1R11; Protein phosphatase 1 regulatory subunit 11; protein phosphatase 1, regulatory (inhibitor) subunit 11; protein phosphatase 1, regulatory (inhibitor) subunit 11, isoform 1; Protein phosphatase inhibitor 3; t-complex-associated-testis-expressed 5; TCTE5; TCTEX5
Product Type Specific: PPP1R11 (HCG V) phosphosite-specific antibody
Antibody Code: PN532
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y64
Protein UniProt: O60927
Protein SigNET: O60927
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human PPP1R11 (HCG V) sequence peptide Cat. No.: PE-04ADI99
Antibody Immunogen Sequence: CCI(pY)EKP(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues C61 to P67; In the PPI_Ypi1 domain. This is the major in vivo phosphorylation site in PPP1R11.
Production Method: Corresponds to amino acid residues C61 to P67; In the PPI_Ypi1 domain. This is the major in vivo phosphorylation site in PPP1R11.
Antibody Modification: PPP1R11 (HCG V) phosphosite-specific antibody
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 18-23 kDa.
Antibody Specificity: High
Antibody Cross Reactivity: Almost no significant cross-reactivities detected in T98G cells.
Related Product 1: PPP1R11-pY64 blocking peptide
Scientific Background: PPP1R11 is a regulatory subunit for protein-serine/threonine phosphatase 1. It inhibits its phosphatase activity. Y64 is amonst the most commonly reported tyrosine phosphorylation sites in human proteins as observed by mass spectrometry. The role of this phosphorylation site is unclear.
