Product Name:
PLCG2-pY759
Product Number:
ab-pn531
Target Full Name: 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-2
Target Alias: 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-2; Phosphoinositide phospholipase C-gamma-2; phospholipase C gamma 2; phospholipase C, gamma 2 (phosphatidylinositol-specific); phospholipase C, gamma 2 (phosphatidylyinositol-specific); Phospholipase C-gamma-2; Phospholipase C-IV; PLC-gamma-2; PLC-IV; PLCG2
Product Type Specific: PLCG2 phosphosite-specific antibody
Antibody Code: PN531
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y759
Protein UniProt: P16885
Protein SigNET: PLCG2
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human PLCG2 (PLC R) sequence peptide Cat. No.: PE-04ADH99
Antibody Immunogen Sequence: SRM(pY)VDP(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues S756 to P762; Near the end of the second SH2 domain in the protein. Y759 phosphorylation stimulates phospholipase activity, and induces interaction with Lyn. This is one of the major in vivo phosphorylation sites in PLCG2 (≥229 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus). This human phosphosite is conserved in many mammals and found in the rat. PLCG2 is known to be phosphorylated at this site in vitro by Btk, Fyn, Lck, Src, and Syk.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus Macaque | Dog | Rat | Mouse.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 140-160 kDa.
Related Product 1: PLCG2 (756-762) pY759 antibody blocking peptide (Cat. No.: PE-04ADH99)
Related Product 2: PLCB3 S1105 phosphorylation antibody (Cat. No.: AB-PN719)
Related Product 3: PLCE1 S1096+T1100 phosphorylation antibody (Cat. No.: AB-PN721)
Related Product 4: PLCG1 Y771+Y775 phosphorylation antibody (Cat. No.: AB-PN774)
Related Product 5: PLCG1 Y783 phosphorylation antibody (Cat. No.: AB-PN530)
Related Product 6: PLCG1 Y977 phosphorylation antibody (Cat. No.: AB-PN722)
Related Product 7: PLCG2 Y1217 phosphorylation antibody (Cat. No.: AB-PN776)
Related Product 8: PLCG2 Y753 phosphorylation antibody (Cat. No.: AB-PN723)
Related Product 9: PLCG2 Y759 phosphorylation antibody (Cat. No.: AB-PN775)
Scientific Background: PLCG2 mediates the production of the second messenger molecules diacylglycerol (DAG) and 1D-myo-inositol 1,4,5-trisphosphate (IP3) from the hydrolysis of 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate. It becomes activated in response to ligand-mediated activation of receptor-type receptor-tyrosine kinases and is calcium-activated. This description may include information annotated by UniProt and/or Google AI.