Product Name:
PLCG1-pY783
Product Number:
ab-pn530
Target Full Name: 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-2
Target Alias: 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma 1; NCKAP3; Phospholipase C, gamma 1; Phospholipase C-gamma-1; PLC1; PLC148; PLC-148; PLCG-1; PLCgamma1; PLC-gamma1; PLC-II
Product Type Specific: Phospholipase phosphosite-specific antibody
Antibody Code: PN530
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y783
Protein UniProt: P19174
Protein SigNET: P19174
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human PLCG1 sequence peptide Cat. No.: PE-04AFD99
Antibody Immunogen Sequence: PGF(pY)VEA(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues P780 to A786; In the intervening region between the second SH2 domain and the SH3 domain in PLCG1. This is one of the major sites of in vivo phosphorylation of PLCG1.
Production Method: Corresponds to amino acid residues P780 to A786; In the intervening region between the second SH2 domain and the SH3 domain in PLCG1. This is one of the major sites of in vivo phosphorylation of PLCG1.
Antibody Modification: Phospholipase phosphosite-specific antibody
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 140-155 kDa.
Related Product 1: PLCG1-pY783 blocking peptide
Related Product 2: PLCG2-pY759 phosphosite-specific antibody (Cat. No.: AB-PN531)
Scientific Background: PLCG1 mediates the production of the second messenger molecules diacylglycerol (DAG) and 1D-myo-inositol 1,4,5-trisphosphate (IP3) from the hydrolysis of 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate. It becomes activated in response to ligand-mediated activation of receptor-type receptor-tyrosine kinases, such as PDGFRA, PDGFRB, FGFR1, FGFR2, FGFR3 and FGFR4. By virtue of its SH2 domains, PLCG1 binds to tyrosine-phosphorylates receptors and become activated by direct tyrosine phosphorylation by these receptors. It participates in actin reorganization and cell migration.
