Product Name:
PLCG1-pY783
Product Number:
ab-pn530
Target Full Name: 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-2
Target Alias: 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma 1; NCKAP3; Phospholipase C, gamma 1; Phospholipase C-gamma-1; PLC1; PLC148; PLC-148; PLCG-1; PLCgamma1; PLC-gamma1; PLC-II
Product Type Specific: PLCG1 phosphosite-specific antibody
Antibody Code: PN530
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y783
Protein UniProt: P19174
Protein SigNET: PLCG1
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human PLCG1 sequence peptide Cat. No.: PE-04AFD99
Antibody Immunogen Sequence: PGF(pY)VEA(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues P780 to A786; in the intervening region between the second SH2 domain and the SH3 domain in PLCG1. Y783 phosphorylation stimulates phospholipase activity, induces interaction with Grb2 and VEGFR-2 and regulates cell motility. This is one of the major sites of in vivo phosphorylation of PLCG1 (≥566 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus). PLCG1 is known to be phosphorylated at this site in vitro by EGFR, Flt1 (VEGFR1), ITK, Ret, Src, Syk, TrkB (NTRK2), and ZAP70.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus Macaque | Dog | Rat | Mouse.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 140-155 kDa.
Related Product 1: PLCG1 (780-786) pY783 antibody blocking peptide (Cat. No.: PE-04AFD99)
Related Product 2: PLCB3 S1105 phosphorylation antibody (Cat. No.: AB-PN719)
Related Product 3: PLCE1 S1096+T1100 phosphorylation antibody (Cat. No.: AB-PN721)
Related Product 4: PLCG1 Y771+Y775 phosphorylation antibody (Cat. No.: AB-PN774)
Related Product 5: PLCG1 Y977 phosphorylation antibody (Cat. No.: AB-PN722)
Related Product 6: PLCG2 Y1217 phosphorylation antibody (Cat. No.: AB-PN776)
Related Product 7: PLCG2 Y753 phosphorylation antibody (Cat. No.: AB-PN723)
Related Product 8: PLCG2 Y759 phosphorylation antibody (Cat. No.: AB-PN531)
Related Product 9: PLCG2 Y759 phosphorylation antibody (Cat. No.: AB-PN775)
Scientific Background: PLCG1 mediates the production of the second messenger molecules diacylglycerol (DAG) and 1D-myo-inositol 1,4,5-trisphosphate (IP3) from the hydrolysis of 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate. It becomes activated in response to ligand-mediated activation of receptor-type receptor-tyrosine kinases, such as PDGFRA, PDGFRB, FGFR1, FGFR2, FGFR3 and FGFR4. By virtue of its SH2 domains, PLCG1 binds to tyrosine-phosphorylates receptors and become activated by direct tyrosine phosphorylation by these receptors. It participates in actin reorganization and cell migration. This description may include information annotated by UniProt and/or Google AI.