Product Name:
PKM2-pY390
Product Number:
ab-pn529
Target Full Name: Pyruvate kinase isozymes M1/M2
Target Alias: CTHBP; Cytosolic thyroid hormone-binding protein; KPYM; MGC3932; OIP-3; OIP3; Opa-interacting protein 3; p58; PK, muscle type; PK2; PK3; PKM; PKM2; Pyruvate kinase 2/3; Pyruvate kinase isozymes M1/M2; Pyruvate kinase muscle isozyme; pyruvate kinase, muscle; TCB; THBP1; Thyroid hormone-binding protein 1; thyroid hormone-binding protein, cytosolic; tumour M2-PK
Product Type Specific: Pyruvate kinase phosphosite-specific antibody
Antibody Code: PN529
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y390
Protein UniProt: P14618
Protein SigNET: P14618
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human PKM2 sequence peptide Cat. No.: PE-04AHE95
Antibody Immunogen Sequence: AAI(pY)HLQ(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues A387 to Q393; At the end of the PK domain. A major phosphorylation site in PKM2.
Production Method: Corresponds to amino acid residues A387 to Q393; At the end of the PK domain. A major phosphorylation site in PKM2.
Antibody Modification: Pyruvate kinase phosphosite-specific antibody
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 57-62 kDa.
Related Product 1: PKM2-pY390 blocking peptide
Scientific Background: PKM2 functions as a glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, and generates ATP. It stimulates POU5F1-mediated transcriptional activation. It appears to plays a general role in caspase independent cell death of tumour cells. The ratio between its highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is thought to be important for tumour cell proliferation and survival.
