Product Name:
PIK3R2-pY464
Product Number:
ab-pn528
Target Full Name: Phosphatidylinositol 3-kinase regulatory subunit beta
Target Alias: p85; p85-BETA; P85B; Phosphatidylinositol 3-kinase 85 kDa regulatory subunit beta; Phosphatidylinositol 3-kinase regulatory subunit beta; phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 2 (p85 beta); phosphoinositide-3-kinase, regulatory subunit 2 (beta); phosphoinositide-3-kinase, regulatory subunit 2 (p85 beta); phosphoinositide-3-kinase, regulatory subunit, polypeptide 2 (p85 beta); PI3-kinase regulatory subunit beta; PI3-kinase subunit p85-beta; PI3K regulatory subunit beta; PIK3R2; PtdIns-3-kinase regulatory subunit beta; PtdIns-3-kinase regulatory subunit p85-beta
Product Type Specific: PIK3R2 phosphosite-specific antibody
Antibody Code: PN528
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y464
Protein UniProt: O00459
Protein SigNET: PIK3R2
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human PIK3R2 sequence peptide Cat. No.: PE-04AFG99
Antibody Immunogen Sequence: DQL(pY)EEY(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues D461 to Y467; in the region between the two SH2 domains in the protein. The effect of Y464 phosphorylation is unclear. This is the major in vivo phosphorylation site in PIK3R2 (≥1687 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1.0 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus Macaque | Dog | Rat | Mouse | Platypus | Chicken | Frog | Zebra fish.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 85-90 kDa.
Antibody Specificity: Very high
Antibody Cross Reactivity: No significant cross-reactive proteins detected in phenylarsine oxide (PAO)+vanadate-treated HeLa cells, EGF-treated A431 cells and insulin-treated MCF7 cells, when these cells were homogenized in SDS-PAGE sample buffer.
Related Product 1: PIK3R2 (461-467) pY464 antibody blocking peptide (Cat. No.: PE-04AFG99)
Related Product 2: PIK3CA Y317 phosphorylation antibody (Cat. No.: AB-PK894)
Related Product 3: PIK3R1 Y467 phosphorylation antibody (Cat. No.: AB-PN526)
Related Product 4: PIK3R1 Y580 phosphorylation antibody (Cat. No.: AB-PN527)
Scientific Background: PIK3R2 is a regulatory subunit of phosphoinositide-3-kinase (PI3K), a kinase that phosphorylates PtdIns(4,5)P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). This phospholipid plays a key role by recruiting PH domain-containing proteins to the membrane, including Akt isoforms and PDPK1, and activates signalling cascades involved in cell growth, survival, proliferation, motility and morphology. This description may include information annotated by UniProt and/or Google AI.