Product Name:
PIK3R1-pY580
Product Number:
ab-pn527
Target Full Name: Phosphatidylinositol 3-kinase regulatory subunit alpha
Target Alias: GRB1; P85; P85A; P85-ALPHA; Phosphatidylinositol 3-kinase regulatory alpha subunit; Phosphoinositide-3-kinase, regulatory subunit 1 (alpha); PI3K; PI3K p85-alpha; PI3-kinase p85-alpha; PtdIns-3-kinase p85-alpha
Product Type Specific: Lipid kinase phosphosite-specific antibody
Antibody Code: PN527
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y580
Protein UniProt: P27986
Protein SigNET: P27986
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human PIK3R1 sequence peptide Cat. No.: PE-04AEX85
Antibody Immunogen Sequence: RDQ(pY)LMW(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues R577 to W583; In the region between the two SH2 domains in the C-terminus half of the protein. This is one of the major in vivo phosphorylation sites inPIK3R1.
Production Method: Corresponds to amino acid residues R577 to W583; In the region between the two SH2 domains in the C-terminus half of the protein. This is one of the major in vivo phosphorylation sites inPIK3R1.
Antibody Modification: Lipid kinase phosphosite-specific antibody
Antibody Concentration: 0.75 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 80-85 kDa.
Related Product 1: PIK3R1-pY580 blocking peptide
Related Product 2: PIK3R1-pY467 phosphosite-specific antibody (Cat. No.: AB-PN526)
Scientific Background: PIK3R1 binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. It is necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. It also mediates cellular signalling in response to various growth factors that bind to other receptor-tyrosine kinases, including EGF, IGF1, VEGFA and PDGF, as well as downstream proteins such as insulin-receptor substrate (IRS) proteins. It plays a role in ITGB2 signalling (PubMed:17626883, PubMed:19805105, PubMed:7518429), and modulates the cellular response to ER stress by promoting nuclear translocation of XBP1 isoform 2 in a ER stress-and/or insulin-dependent manner during metabolic overloading in the liver to improve glucose tolerance (PubMed:20348923).
