Product Name:
PGK1-pY196
Product Number:
ab-pn525
Target Full Name: Phosphoglycerate kinase 1
Target Alias: Cell migration-inducing gene 10 protein; MGC117307; MGC142128; MGC8947; MIG10; PGK1; PGKA; Primer recognition protein 2; PRP 2
Product Type Specific: PGK1 phosphosite-specific antibody
Antibody Code: PN525
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y196
Protein UniProt: P00558
Protein SigNET: P00558
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human PGK1 sequence peptide Cat. No.: PE-04ADF85
Antibody Immunogen Sequence: ELN(pY)FAK(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues E193 to K199; In the middle of the PGK domain. This is the major in vivo phosphorylation site in PGK1.
Production Method: Corresponds to amino acid residues E193 to K199; In the middle of the PGK domain. This is the major in vivo phosphorylation site in PGK1.
Antibody Modification: PGK1 phosphosite-specific antibody
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 44-48 kDa.
Antibody Specificity: Very high
Antibody Cross Reactivity: No significant cross-reactive proteins detected in HEK-293 and T98G cells.
Related Product 1: PGK1-pY196 blocking peptide
Related Product 2: PKG1Subtide - PKG1 (PRKG1) protein kinase substrate peptide
Scientific Background: PGK1 is the second glycolytic enzyme in the pathway to produce pyruvate from glucose, and also acts as a polymerase alpha cofactor protein (primer recognition protein). It has also been implicated to play a role in sperm motility. It catalyzes the reaction: ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate.
