Product Name:
PDLIM5-pY251
Product Number:
ab-pn522
Target Full Name: PDZ and LIM domain protein 5
Target Alias: ENH; ENH1; Enigma homolog; enigma-like LIM domain protein; Enigma-like PDZ and LIM domains protein; L9; LIM; PDLI5; PDLIM5; PDZ and LIM domain 5; PDZ and LIM domain protein 5
Product Type Specific: PDLIM5 phosphosite-specific antibody
Antibody Code: PN522
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y251
Protein UniProt: Q96HC4
Protein SigNET: PDLIM5
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human PDLIM5 (LIM) sequence peptide Cat. No.: PE-04ADC99
Antibody Immunogen Sequence: TEF(pY)HVP(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues T248 to P254; in the middle of the protein between the PDZ and first LIM domains. The effect of Y251 phosphorylation is unclear. This is the major in vivo phosphorylation site of PDLIM5 (≥970 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus Macaque | Dog | Rat | Mouse.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 64-70 kDa.
Antibody Specificity: High-very high
Scientific Background: PDLIM5 (PDZ and LIM domain 5), also known as ENH (Enigma homolog), is a ~63 kDa cytoskeleton-associated scaffold protein that regulates cell morphology, signalling, and structure, particularly in cardiac and neuronal tissues. It possesses an N-terminal PDZ domain and one to three C-terminal LIM domains (cysteine-rich zinc finger domains), allowing it to connect signaling molecules to the cytoskeleton and modulate protein kinase C (PKC) activity. It may play an important role in the heart development by scaffolding PKC to the Z-disk region. It regulates neuronal calcium signaling by interacting with PKC-epsilon and N-type calcium channels. It may play a role in the regulation of cardiomyocyte expansion, since overexpression promotes the development of heart hypertrophy. It also regulates neurodevelopment (dendritic spine morphogenesis). It exists in "long" (with LIM domains) and "short" (lacking LIM domains) forms. The short forms can act differently, often inhibiting myoblast proliferation. Altered PDLIM5 expression is linked to depression, bipolar disorder, and schizophrenia. PDLIM5 is up-regulated in prostate and gastric cancers, and acts as a potential oncoprotein by promoting tumour cell migration and proliferation. This description may include information annotated by UniProt and/or Google AI.