Product Name:
ITSN2-pY968
Product Number:
ab-pn521
Target Full Name: Intersectin-2
Target Alias: ITSN2; KIAA1256; PRO2015; SH3 domain protein 1B; SH3 domain-containing protein 1B; SH3D1B; SH3P18; SH3P18-like WASP associated protein; SH3P18-like WASP-associated protein; SWA; SWAP
Product Type Specific: ITSN2 phosphosite-specific antibody
Antibody Code: PN521
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y968
Protein UniProt: Q9NZM3
Protein SigNET: Q9NZM3
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human ITSN2 sequence peptide Cat. No.: PE-04ADA99
Antibody Immunogen Sequence: EAL(pY)AAV(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues E965 to V971; In the insert region that separates the SH3_2 and SH3_1 domains. This is the second major in vivo phosphorylation site in ITSN2.
Production Method: Corresponds to amino acid residues E965 to V971; In the insert region that separates the SH3_2 and SH3_1 domains. This is the second major in vivo phosphorylation site in ITSN2.
Antibody Modification: ITSN2 phosphosite-specific antibody
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Weak immunoreactivity of a target-sized protein by Western blotting in A431 cells and HepG2 cells. Strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 140-170 kDa.
Antibody Specificity: High
Antibody Cross Reactivity: No significant cross-reactive proteins detected in HepG2 and Jurkat cells.
Related Product 1: ITSN2-pY968 blocking peptide
Scientific Background: ITSN2 is an adapter protein that may provide indirect link between the endocytic membrane traffic and the actin assembly machinery. It may regulate the formation of clathrin-coated vesicles (CCPs), and seems to be involved in CCPs maturation including invagination or budding. ITSN2 is involved in endocytosis of integrin beta-1 (ITGB1) and transferrin receptor (TFR). It also plays a role in dendrite formation by melanocytes.
