Product Name:
HGS-pY216
Product Number:
ab-pn519
Target Full Name: Hepatocyte growth factor-regulated tyrosine kinase substrate
Target Alias: Human growth factor-regulated tyrosine kinase substrate; Protein pp110; Vps27; ZFYVE8
Product Type Specific: HGS phosphosite-specific antibody
Antibody Code: PN519
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y216
Protein UniProt: O14964
Protein SigNET: O14964
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human HGS (Hrs) sequence peptide Cat. No.: PE-04AFB95
Antibody Immunogen Sequence: EPC(pY)EQL(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues E213 to L219; In the FYVE domain. This is the major in vivo phosphorylation site in HGS.
Production Method: Corresponds to amino acid residues E213 to L219; In the FYVE domain. This is the major in vivo phosphorylation site in HGS.
Antibody Modification: HGS phosphosite-specific antibody
Antibody Concentration: 0.2 / 1.0 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 100-110 kDa.
Antibody Specificity: Very high
Antibody Cross Reactivity: No significant cross-reactive proteins detected in phenylarsine oxide (PAO)+vanadate-treated HeLa cells, EGF-treated A431 cells and insulin-treated MCF7 cells, when these cells were homogenized in SDS-PAGE sample buffer.
Related Product 1: HGS-pY216 blocking peptide
Scientific Background: HGS mediates intracellular signal transduction from cytokines and growth factors. When associated with STAM, it suppresses DNA signalling upon stimulation by IL-2 and GM-CSF. It has been implicated as a direct effector of PI3-kinase in vesicular pathway via early endosomes, regulating trafficking to early and late endosomes by recruiting clathrin, concentrating ubiquitinated receptors within clathrin-coated regions, and down-regulating receptor-tyrosine kinases via multivesicular body (MVBs) when complexed with STAM (ESCRT-0 complex). HGS may contribute to the efficient recruitment of SMADs to the activin receptor complex. Involved in receptor recycling via its association with the CART complex.
