Product Name:
HCA59-pY147
Product Number:
ab-pn518
Target Full Name: Uncharacterized protein C9orf78
Target Alias: bA409K20.3; C9orf78; chromosome 9 open reading frame 78; CI078; HCA59; Hepatocellular carcinoma-associated antigen 59; HSPC220
Product Type Specific: HCA59 phosphosite-specific antibody
Antibody Code: PN518
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y147
Protein UniProt: Q9NZ63
Protein SigNET: Q9NZ63
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human HCA59 (Q9NZ63) sequence peptide Cat. No.: PE-04ACZ80
Antibody Immunogen Sequence: DCL(pY)ELP(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues D144 to P150; In the middle of the Hep_59 domain. This is the major in vivo phosphorylation site in HCA59.
Production Method: Corresponds to amino acid residues D144 to P150; In the middle of the Hep_59 domain. This is the major in vivo phosphorylation site in HCA59.
Antibody Modification: HCA59 phosphosite-specific antibody
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 35-39 kDa.
Antibody Specificity: Very high
Antibody Cross Reactivity: No significant cross-reactive proteins detected in A431 cells.
Related Product 1: HCA59-pY147 blocking peptide
Scientific Background: HCA59 is an uncharacterized protein that is known as Hepatocellular carcinoma-associated antigen 59. It is not observed in normal colon tissue. Y147 phosphorylation is amongst the most frequently recorded protein-tyrosine phosphorylation sits by mass spectrometry studies.
