Product Name:
HCA59-pY147
Product Number:
ab-pn518
Target Full Name: Uncharacterized protein C9orf78
Target Alias: bA409K20.3; C9orf78; chromosome 9 open reading frame 78; CI078; HCA59; Hepatocellular carcinoma-associated antigen 59; HSPC220
Product Type Specific: HCA59 phosphosite-specific antibody
Antibody Code: PN518
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y147
Protein UniProt: Q9NZ63
Protein SigNET: HCA59
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human HCA59 (Q9NZ63) sequence peptide Cat. No.: PE-04ACZ80
Antibody Immunogen Sequence: DCL(pY)ELP(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues D144 to P150; in the middle of the Hep_59 domain. The effect of Y147 phosphorylation is unclear. This is the major in vivo phosphorylation site in HCA59 (≥518 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus). HCA59 is known to be phosphorylated at this site in vitro by Syk.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Dog | Mouse | Chicken | Frog.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 35-39 kDa.
Antibody Specificity: Very high
Antibody Cross Reactivity: No significant cross-reactive proteins detected in A431 cells.
Related Product 1: HCA59 (144-150) pY147 antibody blocking peptide (Cat. No.: PE-04ACZ80)
Scientific Background: HCA59 is an uncharacterized protein that is known as Hepatocellular carcinoma-associated antigen 59. It is not observed in normal colon tissue. Y147 phosphorylation is amongst the most frequently recorded protein-tyrosine phosphorylation sits by mass spectrometry studies. This description may include information annotated by UniProt and/or Google AI.